LOCUS       sequence-13199-e        3987 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Yilmaz A, Nishiyama MY Jr, Fuentes BG, Souza GM, Janies D, Gray J, 
            Grotewold E
  TITLE     GRASSIUS: a platform for comparative regulatory genomics across the 
            grasses.
  JOURNAL   Plant Physiol. 2009 Jan;149(1):171-80. doi: 10.1104/pp.108.128579. 
            Epub 2008 Nov 5.
  PUBMED    18987217
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.108.128579"; journalName: "Plant Physiol"; date: 
            "2009-01"; volume: "149"; issue: "1"; pages: "171-80"
COMMENT     Sequence Label: pUT1531-HQ858843
FEATURES             Location/Qualifiers
     source          1..3987
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1544..1643)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1660..1679)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1661..1679)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1684..1700)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1684..1700)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1802..1821)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1813..2622
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1880..1899)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2769..3357
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3258..3277
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3511..3528
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3687..3714)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3806..3892)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3941..3963
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3955..3972
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3956..3972
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      121 atggacatgg acatgagctc ggcttatcct caccattggc tctccttctc cctctctaac
      181 aactaccacc atggcctcct tgaggccctc tctaccacat ctgcacctcc acttggagag
      241 gaggggccag cggagggcgc tccgaagatg gaggatttcc tcggcggcct aggcggaggc
      301 ggcggcgccg tcgccgccgc tccggcagct gccccggagg atcagctcag ctgcggcgag
      361 cttggtagca tcgccgccgg gttcttgcgc cggtacccag cgcctgagaa cgccggcggg
      421 gtgaccatcg caatggcgac cgacgcggcg gcggagctgg ccgatccggc gaggaggacc
      481 gccgagacgt tcgggcaacg gacgtccatc taccgtggtg ccactaggca ccggtggacg
      541 gggaggtacg aggcgcacct gtgggacaat agctgccgcc gggaaggcca aagccgcaaa
      601 ggccgccaag tctacttagg aggttatgat aaggaggaga aggccgcaag agcttacgac
      661 ctcgccgccc taaagtactg gggtccaacc accacgacga acttccctgt tgccaactac
      721 gagacggagc tggaggagat gaagtccatg acgcggcagg agttcatcgc gtcgctgcgc
      781 aggaagagca gcggcttctc aagaggggct tccatctaca gaggagtaac aagacatcat
      841 cagcacggcc ggtggcaagc gaggatcggc agggtggccg gaaacaagga cctgtacttg
      901 ggcactttca gcacgcagga ggaggctgcc gaggcgtacg acattgccgc tatcaagttc
      961 agggggctca acgccgtcac caacttcgac atgagccgct acgacgtcga cagcatcctc
     1021 aacagcgacc tgccggtcgg cggcggagcc gccacgcgcg cctctaagtt cccctctgac
     1081 ccatcgctgc cgctgccgtc gcctgccatg ccaccgtcgg agaaggacta ctggtccctc
     1141 cttgccctgc actaccacca ccaccagcag cagcagcagc agcagcagtt tcctgcttct
     1201 gcatttgaca cctacggctg ctcctccggc gtgaacgtgg acttcacaat ggggactagc
     1261 agccacagcg gcagcaacag caacagcagc agcagcagcg ccatatgggg cacagccgcc
     1321 ggcgcagcga tggggaggca gcaaaacggc ggcagcagca acaagcagag caacagctac
     1381 tccggtaaca acattcctta tgctgctgca gcagctatga cttctggatc agcactctac
     1441 gggggctcca ccggtagcaa cgggacatgg gtggcgagta acacgagcac ggctccccac
     1501 ttctacaact atttgtttgg gatggagaag ggtgggcgcg ccgacccagc tttcttgtac
     1561 aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc
     1621 agtcaaaata aaatcattat ttgccatcca gctgatatcc cctatagtga gtcgtattac
     1681 atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt
     1741 gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat
     1801 acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt aaattccaac
     1861 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
     1921 acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
     1981 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt
     2041 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc
     2101 actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
     2161 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
     2221 tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac
     2281 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
     2341 tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat
     2401 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
     2461 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
     2521 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
     2581 aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg
     2641 ttgtaacact ggcagagcat tacgctgact tgacgggacg gcgcaagctc atgaccaaaa
     2701 tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa
     2761 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     2821 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     2881 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     2941 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     3001 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     3061 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     3121 cgaacgacct acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt
     3181 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     3241 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     3301 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     3361 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     3421 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     3481 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     3541 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     3601 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct
     3661 agccaggaag agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag
     3721 tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca
     3781 caacgttcaa atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac
     3841 aacagataaa acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcctgg
     3901 cagttcccta ctctcgcgtt aacgctagca tggatgtttt cccagtcacg acgttgtaaa
     3961 acgacggcca gtcttaagct cgggccc
//
