LOCUS       sequence-131993-         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV expression of GFP under the Syn promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Boyden ES, Zhang F, Bamberg E, Nagel G, Deisseroth K
  TITLE     Millisecond-timescale, genetically targeted optical control of 
            neural activity.
  JOURNAL   Nat Neurosci. 2005 Sep . 8(9):1263-8.
  PUBMED    16116447
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2005 Sep . 8(9):1263-8."
COMMENT     Sequence Label: pAAV-Syn-GFP
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     218..239
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      281..290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(303..750)
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
ORIGIN
        1 tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg
       61 tagcggctga agcactgcac gccgtaggtc agggtggtca cgagggtggg ccagggcacg
      121 ggcagcttgc cggtggtgca aatgaacttc agggtcagct tgccgtaggt ggcatcgccc
      181 tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg
      241 atgggcacca ccccggtgaa cagctcctcg cccttgctca ccatggtggc ggatcctctc
      301 gactgcgctc tcaggcacga cacgactcct ccgctgccca ccgcagactg aggcagcgct
      361 gagtcgccgg cgccgcagcg cagatggtcg cgcccgtgcc cccctatctc gcgcctcgcg
      421 tggtgcggtc cggctgggcc ggcggcggcg cggacgcgac caaggtggcc gggaagggga
      481 gtttgcgggg gaccggcgag tgacgtcagc gcgccttcag tgctgaggcg gcggtggcgc
      541 gcgccgccag gcgggggcga aggcactgtc cgcggtgctg aagctggcag tgcgcacgcg
      601 cctcgccgca tcctgtttcc cctccccctc tctgataggg gatgcgcaat ttggggaatg
      661 ggggttgggt gcttgtccag tgggtcgggg tcggtcgtca ggtaggcacc cccaccccgc
      721 ctcatcctgg tcctaaaacc cacttgcact catacgcagg gccctctgca gtctagatta
      781 attaaccatt tgctgccgac ccctcccccc aacttctcgg ggactgtggg cgatgtgcgc
      841 tctgcccact gacgggcacc ggagccaatt cccactcctt tcaaga
//
