LOCUS       sequence-131996-         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to construct a gRNA expression cassette for CRISPR/Cas9 genome 
            editing in Marchantia polymorpha.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Sugano SS, Nishihama R, Shirakawa M, Takagi J, Matsuda Y, Ishida S, 
            Shimada T, Hara-Nishimura I, Osakabe K, Kohchi T
  TITLE     Efficient CRISPR/Cas9-based genome editing and its application to 
            conditional genetic analysis in Marchantia polymorpha.
  JOURNAL   PLoS One. 2018 Oct 31;13(10):e0205117. doi: 
            10.1371/journal.pone.0205117. eCollection 2018.
  PUBMED    30379827
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-10-31"; volume: "13(10):e0205117. doi"; pages: " 
            10.1371/journal.pone.0205117. eCollection 2018"
COMMENT     Sequence Label: pMpGE_En01
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    67..91
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt
       61 ataatgccaa ctttgtacaa aaaagcaggc tccgcggccg cccccttcac ctattcattc
      121 aaaagagatt tttaaagatc aatttttctt tctcataagt ctaatacgtc atgataataa
      181 ctttaaaaaa aattgaactc gttaaaaaat ttgatcaaat tttaatttat tattagatca
      241 tatattacat atcagtttca taatgttata ctaagatctt tcatatatat tttaacccac
      301 gagacaaaat atgtttatat ctttatttat ttaaatattt tttagctcaa aacatcgcaa
      361 actcagctat cataatgctt tttaatgaaa aaaacaaatt ttcctatcat actctcattg
      421 tttataattt tttagaaaaa aaacttgaag ataaaataac aacaataata gtttcaaaag
      481 cacatttttg ttgtaaattt caagaaaaat acaatcatat tactgattga ctacaaaaat
      541 ttgtaaatta atccaggcta catgtttaag atgtgtcaaa actgcatcat ctacatctca
      601 ttttaatttt tctaaactac atacatagta tcaataacaa ttgaacaatg ctagttaatg
      661 tgttatactc attagcttgt aatatatctt caacttgatg aatcggattg atcataaaat
      721 ttcacaaact tatgtgctga ttaataaaaa atataatcag atttttcatg aataattaag
      781 ataaataaca catgcaagtc tcaattagaa tttaacaatc tgactataaa aagtcggttt
      841 atcttgattt tcacaaaaat ttaacatagt
//
