LOCUS       sequence-131999-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to construct a gRNA expression cassette for CRISPR/Cas9 genome 
            editing in Marchantia polymorpha.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Sugano SS, Nishihama R, Shirakawa M, Takagi J, Matsuda Y, Ishida S, 
            Shimada T, Hara-Nishimura I, Osakabe K, Kohchi T
  TITLE     Efficient CRISPR/Cas9-based genome editing and its application to 
            conditional genetic analysis in Marchantia polymorpha.
  JOURNAL   PLoS One. 2018 Oct 31;13(10):e0205117. doi: 
            10.1371/journal.pone.0205117. eCollection 2018.
  PUBMED    30379827
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-10-31"; volume: "13(10):e0205117. doi"; pages: " 
            10.1371/journal.pone.0205117. eCollection 2018"
COMMENT     Sequence Label: pMpGE_En03
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    67..91
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_RNA        645..720
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(762..786)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt
       61 ataatgccaa ctttgtacaa aaaagcaggc tccgcggccg cccccttgta acgtgagact
      121 actacactcc tcaacgcaag ttctatctca tatctctgca agagcttatt ccaagaaact
      181 ttcttgtgct tctatgcatg cccaggtacc ttaagtcgcg cgcatagtca cttcacagcc
      241 ttttcagttc tccctttgat ggctcagagt gacgcaactg cacaaaaata agaaaaaagt
      301 cgaagcgtca cgagagatgt atccggctag tttccttgga gttcggattg ctctctttct
      361 ctgtctcccc gaaccatatg gaatttatga actgctgttc actagtcagt cacttgtttc
      421 agaacttttg agtggtcgat ttatccacct tcttgtttct tcctgggtcc agaatctgac
      481 ttcttgagac aacaaacagt gcacagtgga tcgaagaatg accacagttg agacacgtgg
      541 cgctggcctc atttattgca ccatacgttt agtgaagaac tgaataatgg tggatataaa
      601 gcagagttgc acccagcctc tcgggagacc gagagagggt ctcagtttta gagctagaaa
      661 tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc
      721 tttttttcta gtatcgggaa ttctaaaggg tgggcgcgcc gacccagctt tcttgtacaa
      781 agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag
      841 tcaaaataaa
//
