LOCUS       sequence-132-exp        9726 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9726)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1997
REFERENCE   2  (bases 1 to 9726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L2865
FEATURES             Location/Qualifiers
     source          1..9726
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(6911..6930)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6912..6930)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6937..6954)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6937..6953)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6946..6968)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7095..7550
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7182..7201)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7392..7413
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7577..7681
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7682..7750,7751..8542)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAISMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7900..7919)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8713..9301
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9455..9472
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(9625..9630,9631..9648,9649..9655)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9663..9679
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9668..9690
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9687..9703
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9687..9703
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        join(9724..9726,1..16)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 taaccctcac taaagggaac aaaagctggg ggggatcctc caaaatcgtc ttccgctctg
       61 aaaaacgaaa gtggaccttt gacatccgaa aaaatgggcg aaaaaatgaa attgagcttt
      121 ttgggtcgaa aaaaatgttt ttagaatgct gagaacacgt taaacacgaa gatcatattt
      181 attttgagac ccggatgctc tgaaaatgtc tgacatagat ttaaaaaagc atatatatat
      241 ttttcatttt caacgtgaaa gttttgtgca actttataga atctcctatt ggcacattgt
      301 tttttattta actgaggcag tttttgaaca cctttttgaa actttgaatc tctttgaagt
      361 atactgtcga aaagactgac ttgagcgttc gaaatgccag aagaaaacta tatttgaatc
      421 tcgcgctaaa ttgagaaatg caaccgcgct ccactggaca attggaaaaa aaatttattc
      481 ggaggcgaca acggtatttt cgaaattgat tttctgtgta ttttctcatt ttttataaat
      541 tcttctttga tttatcgttc gtttgtgaga aatttaattg tattcaaact tttttatagt
      601 aagataccgg tggtaccgct agccgtacga acccgggttc tagaagcgct gctaaggggg
      661 ccctcgtcga gtcggtcaca atcacctgaa actccaaagg cagccagtga ggaacgtgaa
      721 gaagaagaaa aagagtcatc tgaacaggtt tgattttctt tctggtcaaa aagatgaaat
      781 tattgatttt cagccagata ctcccaaaac tagcagcgag aagtctgcaa gtcgttcaca
      841 gtcgcccaga gaatcgcggg aagtgagcca agaggtatgt ttttcaaaaa tcaataactg
      901 atcataattt ttattgtttg gtgaatttaa gaaaataata ttcgaaaatt cctctgaatt
      961 atcaagattg cagtattaat ttcgagaaaa attgagatat tcatagagct attgtaaatt
     1021 ttcttgattt cagactgaaa cttcggaaaa tcaagagaaa atcaaagaaa aggatgacgg
     1081 ggatgatcag cctggcacac cgaacagcta tagaagccgg gaaacttcac cagctccaaa
     1141 aaggtccaag gagaccaggt ttgtcaaaag cttcctgcga ttaattctca tttcaatttt
     1201 tcagagaatc agagtctcct gaaaaatccc cggttcgttc aagatctccc agaaggtctt
     1261 cagcacgttc cccgtcacga tctcctagac ggcgccgaga aagaagctca gaaagaaagc
     1321 aatccgaaga gccagcaccg ctaccagaga aaaagaagaa agagccgctg gatattctac
     1381 gaacaagaac cggaggagca tatattccac ccgccaaact tcgacttatg caacaacaga
     1441 ttagtgataa gcaaagtgaa cagtatcaga gaatgaattg ggaaagaatg aagaaaaaga
     1501 ttcacggatt ggttaacaga gtcaacgcga agaatcttgt tcaaattgtc agagaacttc
     1561 ttcaagagaa tgtgattcgt tcaaagtgag tgagaaaatc gaaggaaaag gaaagaatta
     1621 atttaatttt tcaggggact tctctgccgt gacattattc aagctcaggc tttctcacca
     1681 ggattctcta acgtctatgc agctttggcg gcagttatca actcgaaatt ccctcatgtc
     1741 ggtgaacttc ttctccgtcg tctgattgta cagttcaaaa gaagtttccg tagaaatgac
     1801 agaggcgtca cggtgaacgt gatcaaattc atcgcacatt tgattaatca acaagttgct
     1861 cacgaagttc ttgcgctgga aatcatgatt ctgatgcttg aagaaccaac tgatgattca
     1921 gttgaagtcg ccattgcgtt cctgaaagag tgtggagcaa agcttctgga gattgctcca
     1981 gcagctctta acagtgtcta cgaccgtctt cgtgcaattc tcatggaaac tgaaagatcg
     2041 gaaaatgcac tggatcgacg tattcagtat atgattgaga ctgcaatgca gattcgaaag
     2101 gacaaatttg cggtaaggta gaatatataa atagtttatt agaaaaaaat aaattagaat
     2161 aatttaaatt cctactagcc aatcaggcga cctttttgcg catagttcta ttattgaaaa
     2221 atttggagaa tttctcatat tctcgctcgg aaatctggaa ttcgacgaga tcttctggct
     2281 tctgtgcagc tgcatcgctt tgtgctccct ttctcgcttg tcttctgtgt acaccaagaa
     2341 ccttgttgag ttcatcaact gaatctgtga ctggcttgtt gctcactgga tgcactagac
     2401 gactgattct cgagaaatca gattgagttg cgattagggt gacctagaaa ttgggaataa
     2461 tacgaacttt tgaaaatatt caggaggatt aaaaaaatta ttctcgacaa tcctacaaat
     2521 ttacttattg caccatgttg ctccaacatt tttcattaaa agttaatgaa aaaatgtaga
     2581 aaatcggaaa ttggcaattt tcagaccatt tttaagcatt ttcaaaaaaa aattgcagct
     2641 gaaataaatg tcattttcag ataaatcgag cgattttctg ttgtctgaca ctagttttta
     2701 gttttaaaaa atgttggaag aacatggtgc aataggtaat ttcatagaat ttccatgtgt
     2761 tttttttcaa ttaaccaatt atccaaatct tccaaactca cattttgcgg agctgggcta
     2821 tcaagaatct gctgcagttt tataagacga gcatctctga tatcactgaa aattaatttt
     2881 taatcaaaac ttgaatatca actaaaccca cttattaact ttctcgatct tctgtcgttc
     2941 ggtacgatga cggtgaagaa gccaattgta gtagttgatt tggttcaagt cctttcggtg
     3001 ttgtacgtca gtgtcctgca atgctattta gttataactt aggcctaaga ttcaatttaa
     3061 tgaagtgatt aaatttgttc tctgaacctc ttaagatgat cttttggatt agaaacatat
     3121 aagacaggtt tacctatcta ttaaaaaaca gatcaaaata gatacgacca aatcggataa
     3181 tccatgccta cctggcatct aggaacgtgt tcttagaaga tttcttacgt aatcgtatga
     3241 agaaataaca atttgatcgt tggccagcaa aaatagggtt ttaagtggga tagtgttttt
     3301 attagctaac cggaaaattt tatagttttt ttttgcaaga aaccactgaa aaccccctaa
     3361 ttgtatacat tttttggagc agcttctggt ctttttgagc aataaaattc gataaaacag
     3421 aatttaagtg taaattgttc acatttagtt tctattttat caaattttgt tgctcaaaaa
     3481 cattcgaagc tgctctaaaa aaatgcatta aaaaaggggt tttcagtggt ttttcacatt
     3541 aaaaaagcta attttaacta aaaatccatc atatttccaa ctttgtcaca acaataaaat
     3601 gctggtcaaa atgtgttcga aaaaatgttt ttttttttaa tttttataat ttaaaaatag
     3661 ttttctttcg ctgggacaca tacatttttg ggcgtaaatt ttcagttcaa atttccattt
     3721 ttacaaccat aatcataaag ctacgtctga tctctctcgc acttacctgc gcctgattcg
     3781 aaagaacaac cgtagccaaa agaacaagaa gaacaagcac gtagttgtgg tagtggacgt
     3841 tcatcacgca atactgacca atggtcgtgg ggtctcactt tccgtactat tgagagaggg
     3901 gagactgaag atggcaattg aggacagtgt cttcgacgca cgcatgcatc cataagcata
     3961 atccaggagg gatggagaga aaaatcttgt ttctaagccc ctccctttgt aatacataca
     4021 catatctaat accgaagaat ggctaattga atggacgtca gctgttgctg tagttgccaa
     4081 ggcatcatcg atgaaataac tgaaagaaag aattaaataa ttattgcagg cgtatccggc
     4141 ggtcattgaa gacttggact tgattgagga ggaggatcag atcatccata cacttaattt
     4201 ggaggatgcg gttgatccgg aaaatgggct tagtaagtga ctgaccacac gcggggggca
     4261 ttaatttaat aaattgaatt ccatttcaga tgtgttcaaa ctagatccag aattcgaaaa
     4321 gaacgaggag gtttatgagg agatccgtaa ggaaatcatt ggaaacgccg atatttcgga
     4381 tgaggatggt ggcgacgagt tggatgatga agaagagggt agtgatgtgg aagaggctcc
     4441 gaagaagact acagagatta ttgataatac tgatcagaat tgactgcttt cagaaggtat
     4501 tcattttgag ttttgggccg gcaaatctgt aagttgccgg ttgccgaaaa tttgctgaat
     4561 ttgccggaaa aaaaaattcc ggaatttatt taaaaacttt ttgtaaaaat taaattaaat
     4621 ttgcaacttt tcagagaagt ctacctgaca atgcaatcat ctttggacta ccaagaagct
     4681 gctcacaaat tgctgaaaat gaagattcca gacagcatgc aggtcagcga tgttgcaaag
     4741 aaaaattttc gaccaaaaaa accaaccaat cataaaattt aaaaaaaaac tccgtttttt
     4801 tctttttttt tatacgagaa aaaccaaaaa aatgtatttt tgccaaattc taaaatacta
     4861 tccccgaaat tttcaatatt ttctctttca gaacgaactc tgcgcgatgc ttgtcgattg
     4921 ttgtgctcaa cagcgtacct acgagcgatt ctacggaatg ctcatcgaac gtttctgccg
     4981 acttcgcctc gaataccagc aatactttga aaagctctgc caggacacgt attccacgat
     5041 tcaccgaatt gacatcacaa aactgcggaa tttggctcgc cttattgctc atttgctctc
     5101 gacggatgct attgactgga agattttggc cgatatgaaa atgaccgaag aggacacaac
     5161 ttcttctggc agaatctata ttaaatatat atttaatgaa cttgtggagg cgatgggaat
     5221 ggttaaactt cattcgagag ttactgatcc gtgagtttcc tagagagagt tgttttcgta
     5281 ttcaattttc cctattttca gaactttggc tcattgcttt gttggattat tcccacgaac
     5341 taatccgaac agcgcacgat tttcgatcaa cttcttcaca atgattggat tgggtggttt
     5401 gacgttggaa cttcgtgaat ggctggcaaa gggtctcaag aagaagaagg gaatgctgga
     5461 tcagttgaag gccgaatcaa gctcagattc atcgtcgtct tcggattcgt cagactcgtc
     5521 tgattcttcg gattctgacg attcatccga ctcgtcttca gattcctcat cttcttcaga
     5581 atcagagcca gaaccaccga agaaaaagaa gaagaagaac agtgaagaga gttccaaaaa
     5641 gaaggaaaaa gagaatattg gtcgacggga tcgtggagac aagagagctg aacgtcatcg
     5701 tgatcaaagt gtggagaaca aggacaagga tcgtcgacgt cgccaggatt ctgacgaaaa
     5761 tcgtcggcca gaacgaggag atgaccgcaa ggatcggagt aaagatcgtc gtcgtcaaga
     5821 ctcggatgat gaggatcgga aaggtcgtga acgtcgggaa gattcagggg aaagacgtcg
     5881 cggagatcgg gatcgacgtg atcgaaacaa ggatcaggag gatcaccgtg aagatcgccg
     5941 tgaccgaagc aaggatcgtg aggatcgacg tgatcgccgt cgtcatgact ctgatgatga
     6001 tcgtaaaact cgtcgggata gaagtgaaga gcgaggagga cgtcgtcgtg aagtggaatc
     6061 ggatgatcga cgccgacgtc gttgaatttt caaattttaa atactgaata tttgtttttt
     6121 ttcctattat ttatttattc tctttgtgtt ttttttcttg ctttctaaaa aattaattca
     6181 atccaaatct aaacattttt ttttctcttt ccgtctccca attcgtattc cgctcctctc
     6241 atctgaacac aatgtgcaag tttatttatc ttctcgcttt catttcatta ggacgtgggg
     6301 ggaattggtg gaagggggaa acacacaaaa ggatgatgga aatgaaataa ggacacacaa
     6361 tatgcaacaa cattcaattc agaaatatgg aggaaggttt aaaagaaaac ataaaaatat
     6421 atagaggagg aaggaaaact agtaaaaaat aagcaaagaa attaggcgaa cgatgagaat
     6481 tgtcctcgct tggcaaatgc gaatccgtat ggagaggcac gtttggcgaa ggcaaatgtt
     6541 cggtatggag atctgtaaaa atttttaagt tgaaatttgg tgttgctctt ttacaaaatt
     6601 ttccgatttt cgcttgaaat tacggtgcca ggtctcgaca cgtcttccaa tttttcaaat
     6661 tcaaaagagc ctttaatggg ctgtagttgc taatttctcg tttttgaaaa tttttcttcc
     6721 gtttaatcga aatttgatgt attttattta tgattttcaa taaatttcaa agaaactggt
     6781 gaaaactcgg aaaattgtga actacagtaa tccaatcctt aaaggcgcac accttttaaa
     6841 tgtccgcccc aatacgatat ttttttaaga ttcgctagag cggccgccac cgcggtggag
     6901 ctccaattcg ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg
     6961 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccccttcgc
     7021 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgtagcct
     7081 gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     7141 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     7201 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     7261 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     7321 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     7381 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     7441 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     7501 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt caggtggcac
     7561 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     7621 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     7681 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     7741 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     7801 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     7861 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     7921 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     7981 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     8041 atgcagtgct gccataagca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     8101 cggaggaccg aaggagctaa ccgctttttt tcacaacatg ggggatcatg taactcgcct
     8161 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     8221 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     8281 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     8341 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     8401 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     8461 cacgacgggc agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     8521 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     8581 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     8641 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     8701 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     8761 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     8821 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     8881 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     8941 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     9001 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     9061 ggagcgaacg acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac
     9121 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     9181 gcgcacgagg gagcttccag gggggaacgc ctggtatctt tatagtcctg tcgggtttcg
     9241 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggccga gcctatggaa
     9301 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     9361 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     9421 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     9481 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     9541 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     9601 cctcactcat taggcacccc aggctttaca ctttatgctt ccggctccta tgttgtgtgg
     9661 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct
     9721 cggaat
//
