LOCUS       sequence-132001-         404 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to construct a gRNA expression cassette for CRISPR/Cas9 genome 
            editing in Marchantia polymorpha.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Sugano SS, Nishihama R, Shirakawa M, Takagi J, Matsuda Y, Ishida S, 
            Shimada T, Hara-Nishimura I, Osakabe K, Kohchi T
  TITLE     Efficient CRISPR/Cas9-based genome editing and its application to 
            conditional genetic analysis in Marchantia polymorpha.
  JOURNAL   PLoS One. 2018 Oct 31;13(10):e0205117. doi: 
            10.1371/journal.pone.0205117. eCollection 2018.
  PUBMED    30379827
REFERENCE   2  (bases 1 to 404)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-10-31"; volume: "13(10):e0205117. doi"; pages: " 
            10.1371/journal.pone.0205117. eCollection 2018"
COMMENT     Sequence Label: pMpGE_En03
FEATURES             Location/Qualifiers
     source          1..404
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    71..95
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_RNA        complement(137..212)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgata agcaatgctt
       61 tcttataatg ccaactttgt acaagaaagc tgggtcggcg cgcccaccct ttagaattcc
      121 cgatactaga aaaaaagcac cgactcggtg ccactttttc aagttgataa cggactagcc
      181 ttattttaac ttgctatttc tagctctaaa actgagaccc tctctcggtc tcccgagagg
      241 ctgggtgcaa ctctgcttta tatccaccat tattcagttc ttcactaaac gtatggtgca
      301 ataaatgagg ccagcgccac gtgtctcaac tgtggtcatt cttcgatcca ctgtgcactg
      361 tttgttgtct caagaagtca gattctggac ccaggaagaa acaa
//
