LOCUS       sequence-132007-        2341 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses spider monkey cytochrome c along with the heme lyase 
            (CYC3) from yeast for heme insertion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2341)
  TITLE     Bowler lab pBTR(SMCytc)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBTR(SMCytc)
FEATURES             Location/Qualifiers
     source          1..2341
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     39..58
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     292..309
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    426..447
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(462..467,468..485,486..492)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    500..516
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     505..527
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     524..540
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     524..540
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     RBS             601..623
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(2287..2304)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2287..2303)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2296..2318)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gggtcggaac aggagagcgc acgagggagc ttccagaggg gaaacgcctg gtatctttat
       61 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
      121 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
      181 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
      241 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
      301 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
      361 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
      421 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
      481 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
      541 catgattacg aattcgagct cgttacccgg ggatccataa ctaactaatc tagaaataat
      601 tttgtttaac tttaagaagg agatatatcc atgggcgacg tggaaaaagg caaacgtatc
      661 ttcatcatga aatgctcgca gtgccacacg gtggaaaaag gcggcaaaca caaaaccggt
      721 cccaacctgc acggcctgtt cggccgcaaa acgggccagg cgtcgggctt cacctacacg
      781 gaagcgaaca aaaacaaagg catcatctgg ggcgaagaca cgctgatgga atacctcgag
      841 aacccgaaaa aatacatccc gggcacgaaa atgatcttcg tgggcatcaa aaaaaaagaa
      901 gaacgcgcgg acctgatcgc gtacctgaaa aaggcgacga acgaatgata aggtaccaag
      961 atggcctttg gtgggttgaa gaaggaaaaa gacagaaacg acttaattac ctacttgaaa
     1021 aaagccactg agtaaacagg ccccttttcc tttgtcgatc ggatccggcc aagctagctt
     1081 ttcagtaatt atctttttag taagctagct aagtttttac acttagttaa attgctaact
     1141 ttatagatta caaaacttag gagggtatcg atactatgaa ttcacaaaaa atgggttggt
     1201 tttgggcaga tcaaaaaact acgggcaaag atattggtgg ggcagcagta tcatccatgt
     1261 cagggtgccc agtcatgcac gagtcgtcgt cgtcgtcgcc accatcctct gagtgccccg
     1321 ttatgcaggg agataacgat agaataaacc cgctgaacaa tatgccggag ttggcagcat
     1381 ccaaacagcc tggccaaaag atggacttgc ccgttgatcg gaccatctcc agcatcccca
     1441 agagtccaga cagtaacgag ttctgggagt atccttctcc acaacagatg tacaatgcta
     1501 tggttagaaa gggcaagatt ggcggtagcg gcgaagtcgc cgaagatgca gtggagtcga
     1561 tggtgcaggt ccacaacttt ctaaatgaag ggtgctggca ggaagtgctc gaatgggaaa
     1621 aaccgcacac agatgaaagc cacgtgcagc ctaagttgct gaaattcatg gggaaaccgg
     1681 gcgtattgag ccctcgtgct cgctggatgc acctgtgcgg cctactgttt ccgtcccatt
     1741 ttagccaaga actaccattc gacaggcacg actggattgt actccgaggc gagcgcaaag
     1801 cggaacaaca acctccaacc ttcaaggaag ttagatacgt cttggatttc tacggagggc
     1861 ccgacgacga aaacggaatg cctactttcc acgtggatgt ccgtcctgcc ctagatagtc
     1921 tagacaatgc taaggaccgg atgacccgtt tcttggaccg gatgatctcg ggtccgtcct
     1981 cttcgtcctc cgccccttaa atgatataca gccagcgtaa gtacgtgtaa atagccaagt
     2041 aagaaataat gatgtcctag tgcagccaca atcaatttca ctttttcata tatatttttc
     2101 caccttatta tcacaaggtg cacctttatc gtgtgccacg gcggtaaaaa actaccttca
     2161 ggggtacgat acattctgtg ctggcgacca cggggctgac agagacaccc gtagaggcta
     2221 cattactgat ttgggaaatt tcccaaattg gaaatatcac ctcgtcgacc tgcagccaag
     2281 cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     2341 t
//
