LOCUS       sequence-132017-         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homologues recombination donor plasmid for inserting a FLAG-SNAP-tag
            at the N-terminus of the TERT locus in human cells. Includes GFP 
            marker flanked by LoxP sites for selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Xi L, Schmidt JC, Zaug AJ, Ascarrunz DR, Cech TR
  TITLE     A novel two-step genome editing strategy with CRISPR-Cas9 provides 
            new insights into telomerase action and TERT gene expression.
  JOURNAL   Genome Biol. 2015 Nov 10;16(1):231. doi: 10.1186/s13059-015-0791-1.
  PUBMED    26553065
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-11-10"; volume: "16(1):231. doi"; pages: " 
            10.1186/s13059-015-0791-1"
COMMENT     Sequence Label: FLAG-LoxP-GFP-LoxP-SNAP-TERT
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     203..224
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(276..605)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      290..425
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     590..610
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     protein_bind    complement(618..651)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(652..717)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac
       61 tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg gcacgggcag cttgccggtg
      121 gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc ctcgccggac
      181 acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg caccaccccg
      241 gtgaacagct cctcgccctt gctcaccatg agctttttgc aaaagcctag gcctccaaaa
      301 aagcctcctc actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa
      361 ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc
      421 gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc
      481 atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga
      541 tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga
      601 cacacattcc aaagcttata acttcgtata gcatacatta tacgaagtta tcttgtcatc
      661 gtcatccttg taatcgatat catgatcttt ataatcaccg tcatggtctt tgtagtccat
      721 cgcgggggtg gccggggcca gggcttccca cgtgcgcagc aggacgcagc gctgcctgaa
      781 actcgcgccg cgaggagagg gcg
//
