LOCUS       sequence-132019-         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homologues recombination donor plasmid for inserting a FLAG-SNAP-tag
            at the N-terminus of the TERT locus in human cells. Includes GFP 
            marker flanked by LoxP sites for selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Xi L, Schmidt JC, Zaug AJ, Ascarrunz DR, Cech TR
  TITLE     A novel two-step genome editing strategy with CRISPR-Cas9 provides 
            new insights into telomerase action and TERT gene expression.
  JOURNAL   Genome Biol. 2015 Nov 10;16(1):231. doi: 10.1186/s13059-015-0791-1.
  PUBMED    26553065
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-11-10"; volume: "16(1):231. doi"; pages: " 
            10.1186/s13059-015-0791-1"
COMMENT     Sequence Label: FLAG-LoxP-GFP-LoxP-SNAP-TERT
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cgatatcgga tgggtgggag tggcgcgtgc cagagagcgc accctccaaa gaggtggctt
       61 cttcggcggg tctggcaggt gacaccacac agaaaccacg gtcactcggt ccacgcgtcc
      121 tgcccgggtg ggcccaggac ccctgcccaa cgggcgtccg ctccggctca ggggcagcgc
      181 cacgcctggg cctcttgggc aacggcagac ttcggctggc actgcccccg cgcctcctcg
      241 cacccggggc tggcaggccc agggggaccc cggcctccct gacgctatgg ttccaggccc
      301 gttcgcatcc cagacgcctt cggggtccac tagcgtgtgg cgggggccgg gcctgagtgg
      361 cagcgccgag ctggtacagc ggcggcccgc acacctggta ggcgcagctg ggagccacca
      421 gcacaaagag cgcgcagcgt gccagcaggt gaaccagcac gtcgtcgccc acgcggcgca
      481 gcagcagccc ccacgccccg ctcccccgca gtgcgtcggt caccgtgttg ggcaggtagc
      541 tgcgcacgct ggtggtgaag gcctcggggg ggcccccgcg ggccccgtcc agcagcgcga
      601 agccgaaggc cagcacgttc ttcgcgccgc gctcgcacag cctctgcagc actcgggcca
      661 ccagctcctt caggcaggac acctgcgggg gaagcgccct gagtcgcctg cgctgctctc
      721 cgcatgtcgc tggttccccc cggccgccct caaccccagc cggacgccg
//
