LOCUS       sequence-132020-         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homologues recombination donor plasmid for inserting a FLAG-SNAP-tag
            at the N-terminus of the TERT locus in human cells. Includes GFP 
            marker flanked by LoxP sites for selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Xi L, Schmidt JC, Zaug AJ, Ascarrunz DR, Cech TR
  TITLE     A novel two-step genome editing strategy with CRISPR-Cas9 provides 
            new insights into telomerase action and TERT gene expression.
  JOURNAL   Genome Biol. 2015 Nov 10;16(1):231. doi: 10.1186/s13059-015-0791-1.
  PUBMED    26553065
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-11-10"; volume: "16(1):231. doi"; pages: " 
            10.1186/s13059-015-0791-1"
COMMENT     Sequence Label: FLAG-LoxP-GFP-LoxP-SNAP-TERT
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    1..49
                     /note="synthetic polyadenylation signal"
     protein_bind    56..89
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             98..643
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="optimized for bacterial expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHEIKLLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYQQLAALAGNPAATAAVKTALSGNPVPILIPCHRVVSSSGAVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
ORIGIN
        1 aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtgg gatccataac
       61 ttcgtataat gtatgctata cgaagttatc cggtaccatg gacaaagatt gcgaaatgaa
      121 acgtaccacc ctggatagcc cgctgggcaa actggaactg agcggctgcg aacagggcct
      181 gcatgaaatt aaactgctgg gtaaaggcac cagcgcggcc gatgcggttg aagttccggc
      241 cccggccgcc gtgctgggtg gtccggaacc gctgatgcag gcgaccgcgt ggctgaacgc
      301 gtattttcat cagccggaag cgattgaaga atttccggtt ccggcgctgc atcatccggt
      361 gtttcagcag gagagcttta cccgtcaggt gctgtggaaa ctgctgaaag tggttaaatt
      421 tggcgaagtg attagctatc agcagctggc ggccctggcg ggtaatccgg cggccaccgc
      481 cgccgttaaa accgcgctga gcggtaaccc ggtgccgatt ctgattccgt gccatcgtgt
      541 ggttagctct agcggtgcgg ttggcggtta tgaaggtggt ctggcggtga aagagtggct
      601 gctggcccat gaaggtcatc gtctgggtaa accgggtctg ggacctgcag gtataggcgc
      661 gccaggatcc ggtaccatgc cgcgcgctcc ccgctgccga gccgtgcgct ccctgctgcg
      721 cagccactac cgcgaggtgc tgccgctggc cacgtt
//
