LOCUS       sequence-132032-         678 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homologues recombination donor plasmid fo the TERT promotor region 
            introducing a SV-40 driven GFP marker in the TERT promotor. Includes
            1kb of TERT coding region in right homologues arm..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Xi L, Schmidt JC, Zaug AJ, Ascarrunz DR, Cech TR
  TITLE     A novel two-step genome editing strategy with CRISPR-Cas9 provides 
            new insights into telomerase action and TERT gene expression.
  JOURNAL   Genome Biol. 2015 Nov 10;16(1):231. doi: 10.1186/s13059-015-0791-1.
  PUBMED    26553065
REFERENCE   2  (bases 1 to 678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-11-10"; volume: "16(1):231. doi"; pages: " 
            10.1186/s13059-015-0791-1"
COMMENT     Sequence Label: HR-TERT-SV40-GFP
FEATURES             Location/Qualifiers
     source          1..678
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    412..460
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(506..527)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgccgaccc cggggaggcc cacctggcgg aaggaggggg cggcgggggg cggccgtgcg
       61 tcccagggca cgcacaccag gcactgggcc accagcgcgc ggaaagccgc cgggtccccg
      121 cgctgcacca gccgccagcc ctggggcccc aggcgccgca cgaacgtggc cagcggcagc
      181 acctcgcggt agtggctgcg cagcagggag cgcacggctc ggcagcgggg agcgcgcggc
      241 atcgcggggg tggccggggc cagggcttcc cacgtgcgca gcaggacgca gcgctgcctg
      301 aaactcgcgc cgcgaggaga gggcggggcc gcggaaagga aggggagggg ctgggagggc
      361 ccggaggggg ctgggccggg gccgcgaggt ccgaagactc atttagatcc tcacacaaaa
      421 aaccaacaca cagatgtaat gaaaataaag atattttatt ttacttgtac agctcgtcca
      481 tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct
      541 cgttggggtc tttgctcagg gcggactggg tgctcaggta gtggttgtcg ggcagcagca
      601 cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt
      661 cctcgatgtt gtggcgga
//
