LOCUS       sequence-132041-         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Cyan Nano-lantern-based luminescent Ca2+ indicator in 
            mammalian cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin 
            T, Okada Y, Nagai T
  TITLE     Expanded palette of Nano-lanterns for real-time multicolor 
            luminescence imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 
            10.1073/pnas.1418468112. Epub 2015 Mar 23.
  PUBMED    25831507
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1418468112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "4352-6"
COMMENT     Sequence Label: Cyan Nano-lantern(Ca2+)/pcDNA3
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(293..370)
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
ORIGIN
        1 ctatagaata gggccctcta gatgcatgct cgagttactg ctcgttcttc agcactctct
       61 ccacgaagct cttgatgtac ttgcccatct cgtcgggggc gtcctcctgg aggaagtgca
      121 ggcccttcac cttcacgaac tcggtgttgg ggaacttctt ggcgccctcc acgatggcgt
      181 tgctgaagaa gccggggtca ccctcgatga acagcttggg caggtcgtcg ctggctctca
      241 ggtaggcgtt gtagtttctc acgatctgca ccacgtcggg cttgccgagc tccagtgccc
      301 cggagctgga gatcttcttg aaccggttgg cagcgctgac ggcaatgaag tttttcttcc
      361 agcgcctctt cccccccttt gctgtcatca tttgtacaaa ctcttcatag tttacttggc
      421 catcaccatc gatatctgct tcccttatca tttcatcaac ttcttcatct gttaacttct
      481 ccccgaggtt tgtcatgacg tgacgtaact gagcagcgct gatgtagccg ttcccatcct
      541 tgtcaaaaac acggaatgct tctcggattt cctcttcgct gtctgtgtcc ttcatttttc
      601 tagccatcat agtaagaaat tcaggaaagt aaatcgttcc attgccatca gcatcgactt
      661 cattgatcat atcctgcaat tctgcttccg ttgggttttg tccaagcgac ctcataacgg
      721 tgccaagttc ctttgtggtg atggtgccgt ccccatcctt gtcgaataat gagaaggctt
      781 ctttgaactc tgcaatctgc tcttctgtca gttggtcatg catccatggg cccttcacca
      841 gggggatctc tctgggccag ctcagggtgg gtcttctcac ctcgcccttc tccttgaagg
      901 gctccaggta
//
