LOCUS       sequence-132065-         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Mito-Cyan Nano-lantern-based luminescent Ca2+ indicator in 
            mammalian cel.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin 
            T, Okada Y, Nagai T
  TITLE     Expanded palette of Nano-lanterns for real-time multicolor 
            luminescence imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 
            10.1073/pnas.1418468112. Epub 2015 Mar 23.
  PUBMED    25831507
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1418468112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "4352-6"
COMMENT     Sequence Label: Mito-Cyan Nano-lantern(Ca2+)/pcDNA3
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(288..365)
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
ORIGIN
        1 gaatagggcc ctctagatgc atgctcgagt tactgctcgt tcttcagcac tctctccacg
       61 aagctcttga tgtacttgcc catctcgtcg ggggcgtcct cctggaggaa gtgcaggccc
      121 ttcaccttca cgaactcggt gttggggaac ttcttggcgc cctccacgat ggcgttgctg
      181 aagaagccgg ggtcaccctc gatgaacagc ttgggcaggt cgtcgctggc tctcaggtag
      241 gcgttgtagt ttctcacgat ctgcaccacg tcgggcttgc cgagctccag tgccccggag
      301 ctggagatct tcttgaaccg gttggcagcg ctgacggcaa tgaagttttt cttccagcgc
      361 ctcttccccc cctttgctgt catcatttgt acaaactctt catagtttac ttggccatca
      421 ccatcgatat ctgcttccct tatcatttca tcaacttctt catctgttaa cttctccccg
      481 aggtttgtca tgacgtgacg taactgagca gcgctgatgt agccgttccc atccttgtca
      541 aaaacacgga atgcttctcg gatttcctct tcgctgtctg tgtccttcat ttttctagcc
      601 atcatagtaa gaaattcagg aaagtaaatc gttccattgc catcagcatc gacttcattg
      661 atcatatcct gcaattctgc ttccgttggg ttttgtccaa gcgacctcat aacggtgcca
      721 agttcctttg tggtgatggt gccgtcccca tccttgtcga ataatgagaa ggcttctttg
      781 aactctgcaa tctgctcttc tgtcagttgg tcatgcatcc atgggccctt caccaggggg
      841 atctctctgg gccagctcag ggtgggtctt ctcacctcgc
//
