LOCUS       sequence-132074-         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Orange Nano-lantern-based luminescent Ca2+ indicator in 
            mammalian cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin 
            T, Okada Y, Nagai T
  TITLE     Expanded palette of Nano-lanterns for real-time multicolor 
            luminescence imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 
            10.1073/pnas.1418468112. Epub 2015 Mar 23.
  PUBMED    25831507
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1418468112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "4352-6"
COMMENT     Sequence Label: Orange Nano-lantern(Ca2+)/pcDNA3
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      5..14
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             11..664
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     CDS             11..664
                     /codon_start=1
                     /product="humanized monomeric Kusabira-Orange fluorescent 
                     protein"
                     /label=hmKO
                     /note="mammalian codon-optimized"
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 gcttgccacc atggtgagcg tgatcaagcc cgagatgaag atgaggtact acatggacgg
       61 ctccgtcaat gggcatgagt tcacaatcga gggtgagggc acaggcagac cttacgaggg
      121 acatcaggag atgacactgc gcgtcacaat ggccgagggc gggccaatgc ctttcgcctt
      181 cgacctggtg tcccacgtgt tctgttacgg ccacagagtt tttactaaat atccagaaga
      241 gatcccagac tatttcaagc aggcctttcc tgagggcctg tcctgggaga ggtccctgga
      301 gttcgaggac ggcggctccg cctccgtgag cgcccacatc agcctgaggg gcaacacctt
      361 ctaccacaag tccaagttca ccggcgtgaa cttccccgcc gacggcccca tcatgcagaa
      421 ccagagcgtg gactgggagc cctccaccga gaagatcacc gccagcgacg gcgtgctgaa
      481 gggcgacgtg accatgtacc tgaagctgga gggcggcggc aaccacaagt gccagatgaa
      541 gaccacctac aaggccgcca aggagatcct ggagatgccc ggcgaccact acatcggcca
      601 caggctggtg aggaagaccg agggcaacat caccgagcag gtggaggacg ccgtggccca
      661 ctccggtacc aaggtgtacg accccgagca acgcaaacgc atgatcactg ggcctcagtg
      721 gtgggctcgc tgcaagcaaa tgaacgtgct ggactccttc atcaactact atgattccga
      781 gaagcacgcc gagaacgccg tgatttttct gcatggtaac gctacctcca gctacctgtg
      841 gaggcacgtc gtgcctcaca tcgagcccgt ggctagatgc atcatccctg atctgatcg
//
