LOCUS       sequence-132075-         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Orange Nano-lantern-based luminescent Ca2+ indicator in 
            mammalian cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin 
            T, Okada Y, Nagai T
  TITLE     Expanded palette of Nano-lanterns for real-time multicolor 
            luminescence imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 
            10.1073/pnas.1418468112. Epub 2015 Mar 23.
  PUBMED    25831507
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1418468112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "4352-6"
COMMENT     Sequence Label: Orange Nano-lantern(Ca2+)/pcDNA3
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(287..364)
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
ORIGIN
        1 aatagggccc tctagatgca tgctcgagtt actgctcgtt cttcagcacg cgctccacga
       61 agctcttgat gtacttaccc atttcatctg gagcgtcctc ctggaggaag tggaggccct
      121 tcaccttcac gaactcggtg ttagggaact tcttagctcc ctcgacaata gcgttggaaa
      181 agaacccagg gtcggactcg atgaacagct taggcagatc gtcgctggcc cgaaggtagg
      241 cgttgtagtt gcggacaatc tggacgacgt cgggcttgcc gagctccagt gccccggagc
      301 tggagatctt cttgaaccgg ttggcagcgc tgacggcaat gaagtttttc ttccagcgcc
      361 tcttcccccc ctttgctgtc atcatttgta caaactcttc atagtttact tggccatcac
      421 catcgatatc tgcttccctt atcatttcat caacttcttc atctgttaac ttctccccga
      481 ggtttgtcat gacgtgacgt aactgagcag cgctgatgta gccgttccca tccttgtcaa
      541 aaacacggaa tgcttctcgg atttcctctt cgctgtctgt gtccttcatt tttctagcca
      601 tcatagtaag aaattcagga aagtaaatcg ttccattgcc atcagcatcg acttcattga
      661 tcatatcctg caattctgct tccgttgggt tttgtccaag cgacctcata acggtgccaa
      721 gttcctttgt ggtgatggtg ccgtccccat ccttgtcgaa taatgagaag gcttctttga
      781 actctgcaat ctgctcttct gtcagttggt catgcatcca tggtccctta acgagaggga
      841 tctcgcgagg ccaggagagg gtaggccgtc taacctcgcc
//
