LOCUS       sequence-132080-        1016 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector of luciferase-eGFP fusion gene driven by Pol2 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Day CP, Carter J, Bonomi C, Esposito D, Crise B, Ortiz-Conde B, 
            Hollingshead M, Merlino G
  TITLE     Lentivirus-mediated bifunctional cell labeling for in vivo melanoma 
            study.
  JOURNAL   Pigment Cell Melanoma Res. 2009 Jun;22(3):283-95. doi: 
            10.1111/j.1755-148X.2009.00545.x. Epub 2009 Jan 19.
  PUBMED    19175523
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1755-148X.2009.00545.x"; journalName: "Pigment Cell 
            Melanoma Res"; date: "2009-06"; volume: "22"; issue: "3"; pages: 
            "283-95"
COMMENT     Sequence Label: pFUGW-Pol2-ffLuc2-eGFP
FEATURES             Location/Qualifiers
     source          1..1016
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(139..155)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(140..156)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    169..193
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(195..908,909..911,912..914))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(198..908,909..911,912..914))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(240..261)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     589..608
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     848..869
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      911..920
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aaggagcaac atagttaaga ataccagtca atctttcaca aattttgtaa tccagaggtt
       61 gattatcgat aagcttgata tcgaattgta cctagtggaa ccggaaccct taaacatgat
      121 ctcgtgaagc gagcttatcg ataccgtcga cctcgagatt ggatcatcac aactttgtac
      181 aagaaagttg gttactactt gtacagctcg tccatgccga gagtgatccc ggcggcggtc
      241 acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac
      301 tgggtgctca ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc
      361 tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag
      421 ttcaccttga tgccgttctt ctgcttgtcg gccatgatat agacgttgtg gctgttgtag
      481 ttgtactcca gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc
      541 tcgatgcggt tcaccagggt gtcgccctcg aacttcacct cggcgcgggt cttgtagttg
      601 ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg
      661 aagaagtcgt gctgcttcat gtggtcgggg tagcggctga agcactgcac gccgtaggtc
      721 agggtggtca cgagggtggg ccagggcacg ggcagcttgc cggtggtgca gatgaacttc
      781 agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg
      841 ccgtttacgt cgccgtccag ctcgaccagg atgggcacca ccccggtgaa cagctcctcg
      901 cccttgctca ccatggtggc gaccggtgga tcccgcacgg cgatcttgcc gcccttcttg
      961 gccttaatga gaatctcgcg gatcttgcgg gcgtccaact tgccggtcag tccttt
//
