LOCUS       sequence-132084-        1018 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector of luciferase-eGFP fusion gene driven by Pol2 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Day CP, Carter J, Bonomi C, Esposito D, Crise B, Ortiz-Conde B, 
            Hollingshead M, Merlino G
  TITLE     Lentivirus-mediated bifunctional cell labeling for in vivo melanoma 
            study.
  JOURNAL   Pigment Cell Melanoma Res. 2009 Jun;22(3):283-95. doi: 
            10.1111/j.1755-148X.2009.00545.x. Epub 2009 Jan 19.
  PUBMED    19175523
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1755-148X.2009.00545.x"; journalName: "Pigment Cell 
            Melanoma Res"; date: "2009-06"; volume: "22"; issue: "3"; pages: 
            "283-95"
COMMENT     Sequence Label: pFUGW-Pol2-ffLuc2-eGFP
FEATURES             Location/Qualifiers
     source          1..1018
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    126..243
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    322..342
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
       61 gattagtgaa cggatcggca ctgcgtgcgc caattctgca gacaaatggc agtattcatc
      121 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
      181 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
      241 tttcgggttt attacaggga cagcagagat ccagtttggt tagtaccggg cccgctctag
      301 attgaattca agggatcatc acaactttgt atagaaaagt tggcaagctt gggcttgaac
      361 atcgagcgcc agggctccgt aaagctacta gagcacaggc ggtgccccaa cgtcctgggg
      421 cctctccact aataacggct acttccaatt gattggacgc gccatcttgc ctgccttatg
      481 catattcagc ggtgaactga atattcatga acgaggcccg tcccgtccct ccctccttcc
      541 ccccaccccc ggaacccgct ccggaggacc cgaagggccc cgccttcatt accgatgcgt
      601 aggacaaacc attttcccga tgtgtgtggg gggatactaa tgagagactt tagctgaaaa
      661 tgagcctgaa ctccgaagct gagtaaaaat ggcctaactt tatcctccgt tctgtaagtc
      721 ctcggtttga gtgcacggga aacccgaaag gaggacgaca ggaccaggaa cattctcctc
      781 ctcctgtcgc gtcagaaaga acacccaacc agggagccgg agccctagcg tcaacaactc
      841 cgccgcgcgc gctccgtgta ggccggtgcg ggcggccccg tagcgcaagg gagggcggga
      901 aaggaagggg cgggacacaa gggcgaatct ataaagggcg tcactcagcc agttctctcc
      961 tcagaagcgc cgagagcgcg accgggacgg ttggagaaga aggtggctcc cggaaggg
//
