LOCUS       sequence-132093-         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Nuc-Orange Nano-lantern-based luminescent Ca2+ indicator in 
            mammalian cel.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin 
            T, Okada Y, Nagai T
  TITLE     Expanded palette of Nano-lanterns for real-time multicolor 
            luminescence imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 
            10.1073/pnas.1418468112. Epub 2015 Mar 23.
  PUBMED    25831507
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1418468112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "4352-6"
COMMENT     Sequence Label: Nuc-Orange Nano-lantern(Ca2+)/pcDNA3
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     31..50
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        31..49
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      63..72
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             69..722
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     CDS             69..722
                     /codon_start=1
                     /product="humanized monomeric Kusabira-Orange fluorescent 
                     protein"
                     /label=hmKO
                     /note="mammalian codon-optimized"
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 agaacccact gcttactggc ttatcgaaat taatacgact cactataggg agacccaagc
       61 ttgccaccat ggtgagcgtg atcaagcccg agatgaagat gaggtactac atggacggct
      121 ccgtcaatgg gcatgagttc acaatcgagg gtgagggcac aggcagacct tacgagggac
      181 atcaggagat gacactgcgc gtcacaatgg ccgagggcgg gccaatgcct ttcgccttcg
      241 acctggtgtc ccacgtgttc tgttacggcc acagagtttt tactaaatat ccagaagaga
      301 tcccagacta tttcaagcag gcctttcctg agggcctgtc ctgggagagg tccctggagt
      361 tcgaggacgg cggctccgcc tccgtgagcg cccacatcag cctgaggggc aacaccttct
      421 accacaagtc caagttcacc ggcgtgaact tccccgccga cggccccatc atgcagaacc
      481 agagcgtgga ctgggagccc tccaccgaga agatcaccgc cagcgacggc gtgctgaagg
      541 gcgacgtgac catgtacctg aagctggagg gcggcggcaa ccacaagtgc cagatgaaga
      601 ccacctacaa ggccgccaag gagatcctgg agatgcccgg cgaccactac atcggccaca
      661 ggctggtgag gaagaccgag ggcaacatca ccgagcaggt ggaggacgcc gtggcccact
      721 ccggtaccaa ggtgtacgac cccgagcaac gcaaacgcat gatcactggg cctcagtggt
      781 gggctcgctg caagcaaatg aacgtgctgg actccttcat caactactat gattccgaga
      841 agcacgccga gaacgccgtg atttttctgc atggtaacgc tacctccagc tacctgtgga
      901 ggcacgtcgt gcctcacatc gagcccgtgg ctagatgcat catc
//
