LOCUS       sequence-132096-         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Nuc-Orange Nano-lantern-based luminescent Ca2+ indicator in 
            mammalian cel.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin 
            T, Okada Y, Nagai T
  TITLE     Expanded palette of Nano-lanterns for real-time multicolor 
            luminescence imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 
            10.1073/pnas.1418468112. Epub 2015 Mar 23.
  PUBMED    25831507
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1418468112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "4352-6"
COMMENT     Sequence Label: Nuc-Orange Nano-lantern(Ca2+)/pcDNA3
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(670..747)
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
ORIGIN
        1 atagggccct ctagatgcat gctcgagtta cttagcgctg gtgtacttgg tgacggcctt
       61 ggtgccctcg gacacggcgt gcttggccaa ctccccgggc agcagcaggc gcacggccgt
      121 ctggatctcc ctggaggtga tggtcgagcg cttgttgtaa tgcgccaggc gggaagcctc
      181 acccgcgatg cgttcgaaga tgtcgttgac gaaggagttc atgattccca tggccttaga
      241 ggagatgccg gtgtcggggt ggacctgctt cagcaccttg tacacgtata cggagtagct
      301 ctccttgcgg ctgcgcttgc gcttcttgcc gtccttcttc tgcgccttag tcacggcttt
      361 cttcgagccc ttcttgggcg cgggagcaga cttcgctggc tccggcatga attcctgctc
      421 gttcttcagc acgcgctcca cgaagctctt gatgtactta cccatttcat ctggagcgtc
      481 ctcctggagg aagtggaggc ccttcacctt cacgaactcg gtgttaggga acttcttagc
      541 tccctcgaca atagcgttgg aaaagaaccc agggtcggac tcgatgaaca gcttaggcag
      601 atcgtcgctg gcccgaaggt aggcgttgta gttgcggaca atctggacga cgtcgggctt
      661 gccgagctcc agtgccccgg agctggagat cttcttgaac cggttggcag cgctgacggc
      721 aatgaagttt ttcttccagc gcctcttccc cccctttgct gtcatcattt gtacaaactc
      781 ttcatagttt acttggccat caccatcgat atctgcttcc cttatcattt catcaacttc
      841 ttcatctgtt aacttctccc cga
//
