LOCUS       sequence-132129-        1072 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway ORF clone of human TSC2 [NM_001114382.1] with stop codon 
            (for native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1072)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E355 Hs.TSC2
FEATURES             Location/Qualifiers
     source          1..1072
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    85..184
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ggtgtgcgtc acccggcaac cttgggtagc agcgaagtcg aggcatttct gtcctggctg
       61 gtctagagga tctaagcttg catgcaaata atgattttat tttgactgat agtgacctgt
      121 tcgttgcaac aaattgataa gcaatgcttt cctataatgc caactttgta caagaaagtt
      181 gattacacaa actcggtgaa gtcctccacc gaggagatga ggcgcttccg ctggcccacc
      241 tcatagccag gtgtgggctc ggctggagtc tgtgcagggg ctttgctatg ggacggaggg
      301 tgcaccagag gtaggctggg gttggagtag gcggcttcct cgcagatccg ctggcggagc
      361 cgcttgatgt ggcggagccg ggcaatccac ttggaggggt agatatcggt ggggttggag
      421 cggctatgat gcacctgtga ggccatattt gcgtgcaggg ccatctggcg ggccacgaag
      481 ggcaggttgc ggtcagacac gatcttggcc acgctggtgt ccacaaggcc ctccatgtct
      541 ttcctgcact gcagggacac caggttgcac tcgtagtcca gcggggtgac gatcacgtgg
      601 acaaagttga actggccctt gatggtgcca agcttgaagt cctcaccgga gtcattgtag
      661 acaatggaca caaagtcgtt gcccaggtgg cgcttcttgt cgcagcggtg cttgtccacg
      721 tccttggtgg gcatcagggt ggcgatgtgg aagacggctt gcatgatgtc atcgtgccag
      781 cagtaggtga actggccgtc ctcaccacac acgtccaggc ctcccaggta caccttgtcc
      841 ggctggcagt ccttcagctc gatgagccgg cccaggcccg tcaggaactc cgtgtacctg
      901 taggagccat gctcattgga caggatggcg agctcgctgt tgctctggcc ttctccaaca
      961 tacaggacgg cgatcttgtg ggtgtcgtat gatgggatct ggtcgaggag ctgcaccgac
     1021 cgctcaaagg actgtgactc attgggcagc aggattggct tgtttgactc gt
//
