LOCUS       sequence-132133-        1127 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway ORF clone of human TSC2 [NM_001114382.1] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1127)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E356 Hs.TSC2-nostop
FEATURES             Location/Qualifiers
     source          1..1127
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    85..184
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ggtgtgcgtc acccggcaac cttgggtagc agcgaagtcg aggcatttct gtcctggctg
       61 gtctagagga tctaagcttg catgcaaata atgattttat tttgactgat agtgacctgt
      121 tcgttgcaac aaattgataa gcaatgcttt cctataatgc caactttgta caagaaagtt
      181 gacacaaact cggtgaagtc ctccaccgag gagatgaggc gcttccgctg gcccacctca
      241 tagccaggtg tgggctcggc tggagtctgt gcaggggctt tgctatggga cggagggtgc
      301 accagaggta ggctggggtt ggagtaggcg gcttcctcgc agatccgctg gcggagccgc
      361 ttgatgtggc ggagccgggc aatccacttg gaggggtaga tatcggtggg gttggagcgg
      421 ctatgatgca cctgtgaggc catatttgcg tgcagggcca tctggcgggc cacgaagggc
      481 aggttgcggt cagacacgat cttggccacg ctggtgtcca caaggccctc catgtctttc
      541 ctgcactgca gggacaccag gttgcactcg tagtccagcg gggtgacgat cacgtggaca
      601 aagttgaact ggcccttgat ggtgccaagc ttgaagtcct caccggagtc attgtagaca
      661 atggacacaa agtcgttgcc caggtggcgc ttcttgtcgc agcggtgctt gtccacgtcc
      721 ttggtgggca tcagggtggc gatgtggaag acggcttgca tgatgtcatc gtgccagcag
      781 taggtgaact ggccgtcctc accacacacg tccaggcctc ccaggtacac cttgtccggc
      841 tggcagtcct tcagctcgat gagccggccc aggcccgtca ggaactccgt gtacctgtag
      901 gagccatgct cattggacag gatggcgagc tcgctgttgc tctggccttc tccaacatac
      961 aggacggcga tcttgtgggt gtcgtatgat gggatctggt cgaggagctg caccgaccgc
     1021 tcaaaggact gtgactcatt gggcagcagg attggcttgt ttgactcgtc gccaaagaag
     1081 ggggaatggt agagctgcag gaacacgaaa ctggggttga tgcctgg
//
