LOCUS       sequence-132153-        1026 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector of luciferase-eGFP fusion gene driven by FerH 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Day CP, Carter J, Bonomi C, Esposito D, Crise B, Ortiz-Conde B, 
            Hollingshead M, Merlino G
  TITLE     Lentivirus-mediated bifunctional cell labeling for in vivo melanoma 
            study.
  JOURNAL   Pigment Cell Melanoma Res. 2009 Jun;22(3):283-95. doi: 
            10.1111/j.1755-148X.2009.00545.x. Epub 2009 Jan 19.
  PUBMED    19175523
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1755-148X.2009.00545.x"; journalName: "Pigment Cell 
            Melanoma Res"; date: "2009-06"; volume: "22"; issue: "3"; pages: 
            "283-95"
COMMENT     Sequence Label: pFUGW-FerH-ffLuc2-eGFP
FEATURES             Location/Qualifiers
     source          1..1026
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    126..243
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    322..342
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        388..579
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(411..431)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
       61 gattagtgaa cggatcggca ctgcgtgcgc caattctgca gacaaatggc agtattcatc
      121 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
      181 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
      241 tttcgggttt attacaggga cagcagagat ccagtttggt tagtaccggg cccgctctag
      301 attgaattca agggatcatc acaactttgt atagaaaagt tggcggcctg aaataacctc
      361 tgaaagagga acttggttag gtaccttctg aggctgaaag aaccagctgt ggaatgtgtg
      421 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
      481 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
      541 gcaaagcatg catctcaatt agtcagcaac catagtccca ctagttccgc cagagcgcgc
      601 gagggcctcc agcggccgcc cctcccccac agcaggggcg gggtcccgcg cccaccggaa
      661 ggagcgggct cggggcgggc ggcgctgatt ggccggggcg ggcctgacgc cgacgcggct
      721 ataagagacc acaagcgacc cgcagggcca gacgttcttc gccgaagctt gccgtcagaa
      781 cgcaggtgag gggcgggtgt ggcttccgcg ggccgccgag ctggaggtcc tgctccgagc
      841 gggccgggcc ccgctgtcgt cggcggggat tagctgcgag cattcccgct tcgagttgcg
      901 ggcggcgcgg gaggcagagt gcgaggccta gcggcaaccc cgtagcctcg cctcgtgtcc
      961 ggcttgaggc ctagcgtggt gtccgcgccg ccgccgcgtg ctactccggc cgcactctgg
     1021 tctttt
//
