LOCUS       sequence-132167-         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pH-Sensitive Red Fluorescent Protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Fan Y, Chen Z, Ai HW
  TITLE     Monitoring redox dynamics in living cells with a redox-sensitive red
            fluorescent protein.
  JOURNAL   Anal Chem. 2015 Mar 3;87(5):2802-10. doi: 10.1021/ac5041988. Epub 
            2015 Feb 20.
  PUBMED    25666702
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ac5041988";
            journalName: "Anal Chem"; date: "2015-03-3- 3"; volume: "87"; issue:
            "5"; pages: "2802-10"
COMMENT     Sequence Label: pcDNA3-pHRFP
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             28..45
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..81
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(85..93,94..108)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 aagcttgccg ccaccatggg aggttctcat catcatcatc atcatggtat ggctagcatg
       61 actggtggac agcaaatggg tcgggatctg tacgacgatg acgataagga tccgagctcg
      121 agcgttcatg ctataggtcg gctgagctca cccgtggttt ccgagcggat gtaccccgag
      181 gacggcgccc tgaagagcga gatcaagaag gggctgaggc tgaaggacgg cggccactac
      241 gccgccgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac
      301 atcgtcgaca tcaagttgga catcgtgtcc cacaacgagg actacaccat cgtggaacag
      361 tgcgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagggaggt
      421 acaggcggga gtctggtgag caagggcgag gaggataaca tggccatcat caaggagttc
      481 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      541 gagggcgagg gccgccccta cgaggccttt cagaccgcta agctgaaggt gaccaagggt
      601 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
      661 tacattaagc acccagccga catccccgac tacttcaagc tgtccttccc cgagggcttc
      721 aggtgggagc gcgtgatgaa cttcgaggac ggcggcatta ttcacgttaa ccaggactcc
      781 tccctgcagg acggcgtatt catctacaag gtgaagctgc gcggcaccaa cttccccccc
      841 gacggccctg taatgcagaa gaagaccatg ggctgggagg ccacgcgtga ccaaaggtcg
      901 tattaagctg gaattc
//
