LOCUS       sequence-132178-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV transfer vector with mammalian codon-optimized ChD-citrine 
            between AAV2 ITR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Lin JY, Lin MZ, Steinbach P, Tsien RY
  TITLE     Characterization of engineered channelrhodopsin variants with 
            improved properties and kinetics.
  JOURNAL   Biophys J. 2009 Mar 4;96(5):1803-14. doi: 10.1016/j.bpj.2008.11.034.
  PUBMED    19254539
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bpj.2008.11"; journalName: "Biophys J"; date: "2009-03-4-
            4"; volume: "96"; issue: "5"; pages: "1803-14"
COMMENT     Sequence Label: AAV-oChD-citrine
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     99..120
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc
       61 ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag
      121 gatgggcacc accccggtga acagctcctc gcccttgctc accatctcga gcaatggtgg
      181 cgacggtgcc acggctccgg cctcggcttc gtcttcgacg agagtctcga cctcgatctc
      241 cgttccgccg atgttcagtt tggtggtttt gcggatatct ccgtgaatca atatgtgctc
      301 gtggatcagg acgcgcaggt agtgtcccaa caacccccaa caatttttac tcatcagatc
      361 aataatcgtg tgacctacgg tggagccata gacgctcagg acgccaaaac cttcgggccc
      421 caaaatgaag agaattggga acatacccca gctcacgaaa aacagccatg ccatgccggt
      481 cacgacctgg cggcaccgac cctttggcac agtatgataa ccctcgatat atgctttggc
      541 ggcgtgaaaa aatgtgttcg cgccatagca caatccaaga caaaagaaga tgactttaac
      601 atagccggtt gccatggcgc tggtagcccc ccacacgatg gtgccgatgt cgctcaccag
      661 caggcccatg gttctcttgt tgtagtcgtt ggccaggccg gtcaggttgc tcaggtggat
      721 caggatcacg gggcaggtca gcagccactc ggcgtatctc agccacacgg tcttgttgcc
      781 gttgctgctg tagatcacgg cgggctcgtc gaactcgtgg aagtactcta tgatgaactt
      841 gatcatctcg
//
