LOCUS       sequence-132183-         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV transfer vector with mammalian codon-optimized ChIEF-citrine 
            between AAV2 ITR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Lin JY, Lin MZ, Steinbach P, Tsien RY
  TITLE     Characterization of engineered channelrhodopsin variants with 
            improved properties and kinetics.
  JOURNAL   Biophys J. 2009 Mar 4;96(5):1803-14. doi: 10.1016/j.bpj.2008.11.034.
  PUBMED    19254539
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bpj.2008.11"; journalName: "Biophys J"; date: "2009-03-4-
            4"; volume: "96"; issue: "5"; pages: "1803-14"
COMMENT     Sequence Label: AAV-oChIEF-citrine
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     99..120
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc
       61 ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag
      121 gatgggcacc accccggtga acagctcctc gcccttgctc accatctcga ggctgctctc
      181 gtacttgccg gtgcccttgt tcacggcgcc ggcctcggcc tcgtcctcca ccagggtctc
      241 cacctcgatc tcggtgccgc cgatgttcag cttggtggtc tttctgatgt cgccgtggat
      301 caggatgtgc tcgtggatca gcactctcag gtagtggccc agcaggcccc agcagttctt
      361 gctcatcagg tcgatgatgg tgtggcccac ggtgctgccg tacacgctca gcacgccgaa
      421 gccctcgggg cccaggatga acaggatggg gaacatgccc cagctcacga agaacagcca
      481 ggccatgccg gtcaccacct gtctgcatct gcccttgggc acggtgtggt aggcctcgat
      541 gtacaccttg gcggcgttga agaaggtgta gatgccgtag cacaggccca tcaggaagaa
      601 gatcactctc acgtagccct tgctcagggc ggcggtggtg ccccacacga tggtgccgat
      661 gtcgctcacc agcaggccca tggttctctt gttgtagtcg ttggccaggc cggtcaggtt
      721 gctcaggtgg atcaggacca cggggcaggt cagcagccac tcggcgtatc tcagccacac
      781 gg
//
