LOCUS       sequence-132189-         671 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV2 transfer vector containing the InSynC (SYP1-miniSOG-citrine) 
            construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Lin JY, Sann SB, Zhou K, Nabavi S, Proulx CD, Malinow R, Jin Y, 
            Tsien RY
  TITLE     Optogenetic inhibition of synaptic release with chromophore-assisted
            light inactivation (CALI).
  JOURNAL   Neuron. 2013 Jul 24;79(2):241-53. doi: 10.1016/j.neuron.2013.05.022.
  PUBMED    23889931
REFERENCE   2  (bases 1 to 671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2013.05"; journalName: "Neuron"; date: 
            "2013-07-24- 24"; volume: "79"; issue: "2"; pages: "241-53"
COMMENT     Sequence Label: AAV-SYP1-miniSOG-citrine
FEATURES             Location/Qualifiers
     source          1..671
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     99..120
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(205..522)
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
ORIGIN
        1 gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc
       61 ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag
      121 gatgggcacc accccggtga acagctcctc gcccttgctc accatatcga ttcccgcgcc
      181 tcctcctcct gagcctcctc ctgatccatc cagctgcact ccgatgaaat actggagctc
      241 acccttctgg tccctcatgg gctggaggtg caggaggttc cagaatttct tcccgctttt
      301 tgtgtagttt atcagctgca cggtaatctc gcgctgatct ctaatggcat cgcgaatctt
      361 ctgcactgtg gcctggtctg tctctggtcc ctgcagaaag cggccattgc ggcccaggat
      421 ctcctctctg gaatactcgg tcagctccag gaagccatcg gatgcgaaga tgatgggatt
      481 gtctggcagc cgtggatcag ttatcacgaa acttttctcc atgctgccgc caccactgcc
      541 gccaccaccg gttttcatct gattggagaa ggaggtgggc gcaccctgtt ggccatagcc
      601 ttgctgccca tagtcgccct gaggcccgta gccaccgcca ccgctggctg gctgcccgta
      661 atcgggttga t
//
