LOCUS       sequence-13219-e        3885 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Yilmaz A, Nishiyama MY Jr, Fuentes BG, Souza GM, Janies D, Gray J, 
            Grotewold E
  TITLE     GRASSIUS: a platform for comparative regulatory genomics across the 
            grasses.
  JOURNAL   Plant Physiol. 2009 Jan;149(1):171-80. doi: 10.1104/pp.108.128579. 
            Epub 2008 Nov 5.
  PUBMED    18987217
REFERENCE   2  (bases 1 to 3885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.108.128579"; journalName: "Plant Physiol"; date: 
            "2009-01"; volume: "149"; issue: "1"; pages: "171-80"
COMMENT     Sequence Label: pUT1672-HQ858863
FEATURES             Location/Qualifiers
     source          1..3885
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             112..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(1442..1541)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1558..1577)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1559..1577)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1582..1598)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1582..1598)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1700..1719)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1711..2520
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1778..1797)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2667..3255
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3156..3175
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3409..3426
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3585..3612)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3704..3790)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3839..3861
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3853..3870
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3854..3870
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac
      121 tttaagaagg agcccttcac catggacatg agcgagagca gtgagaaagg aatggagagc
      181 aatgcgtcgt ctggccctgg caatggcatc ccggtggagt ggcagagcca attcagcagc
      241 gccttcgcgt gccagccgtc ggtggcggcg cagcaccagc agcacgccat gatggactcc
      301 ttcgcggcgg cgtccgccgg cctgtgggcg tcctccgacg tcgtcagcgc catgtcatcg
      361 gccgcgccgc cgcggggcgc ggggttcttg gcgccggtgc ccgggttcct ccagcagggg
      421 ctcggccatt tcccggtgga ctccggcttc atcgagcgcg cagcgcggtc gacgtgcttc
      481 ggcggcggga tgatggccgg cggcccatac ggcgcagctg accaggccat gggcgacgct
      541 ttcggtggca ccgcggaggg tctcatggat catcacagga acgttggcaa cgacaaggcc
      601 gaggagttcg ccggcaacgg ccacgacgag gtgccgagct cggaggtagc cggtggagac
      661 tgctcctcca aaggttcaga ctccaagaag aggagaaggc ctaacgaggt gatgggaacc
      721 gatcaagtcc attcttccaa cttgccatct gattctgcga acgagagtgt tcacagcaaa
      781 gacaaaggcg aggagagcag cccggcgacg acgaacggcg gcaagtcgaa agggaaggga
      841 gcaaaggaga cctctgagtc acagaaagaa gagtacattc atgtccgggc tcggcgtggc
      901 caggcaacca acagccacag cttagcagaa aggttgagaa gggagaagat cagtgagagg
      961 atgaaacttc ttcaggacct agttcctggc tgcagcaagg tcaccggaaa agcggtgatg
     1021 cttgacgaga tcatcaacta tgttcagtcc ctgcaaagac aagttgagtt cttatcgatg
     1081 aagcttgcga cagtaaaccc aaggcttgac ctcaacatag aagggcttct ttcaaaagac
     1141 cttcttcgct tccctggagt tccttcatcc tctatcggat tctccccaga aatgatgcat
     1201 cctcaactac aattgtcaca accaggactg attcatggtg gtaccgctgg catggccaac
     1261 cccgatgtgt tcagacgaat aatacaagcg caattaggtg caaaggatgg atctcagatg
     1321 ccccactcat tgaatggatc attcagtgac gtttcccaaa tggcgtaccc gtccctcggt
     1381 tctcaggacc taagcatcag gccatctcag gatgggtttc aaatgaaggg tgggcgcgcc
     1441 gacccagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc
     1501 aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc
     1561 tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa
     1621 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct
     1681 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg
     1741 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat
     1801 gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg
     1861 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta
     1921 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat
     1981 gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa
     2041 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat
     2101 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg
     2161 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc
     2221 tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca
     2281 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata
     2341 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
     2401 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt
     2461 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa
     2521 tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc
     2581 gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac
     2641 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     2701 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     2761 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     2821 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     2881 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     2941 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     3001 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat
     3061 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     3121 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     3181 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     3241 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     3301 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     3361 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     3421 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     3481 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     3541 attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca
     3601 ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc
     3661 ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga
     3721 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc
     3781 gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc
     3841 cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccc
//
