LOCUS       sequence-132192-         581 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV2 transfer vector containing the InSynC 
            (miniSOG-VAMP2-T2A-mCherry) construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Lin JY, Sann SB, Zhou K, Nabavi S, Proulx CD, Malinow R, Jin Y, 
            Tsien RY
  TITLE     Optogenetic inhibition of synaptic release with chromophore-assisted
            light inactivation (CALI).
  JOURNAL   Neuron. 2013 Jul 24;79(2):241-53. doi: 10.1016/j.neuron.2013.05.022.
  PUBMED    23889931
REFERENCE   2  (bases 1 to 581)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2013.05"; journalName: "Neuron"; date: 
            "2013-07-24- 24"; volume: "79"; issue: "2"; pages: "241-53"
COMMENT     Sequence Label: AAV2-miniSOG-VAMP2-T2A-mCherry
FEATURES             Location/Qualifiers
     source          1..581
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(123..176)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg agccctccat gtgcaccttg
       61 aagcgcatga actccttgat gatggccatg ttatcctcct cgcccttgct caccatcgta
      121 cgagggccgg gattctcctc cacgtcaccg catgttagaa gacttcctct gccctcaccg
      181 ccaccagatc cacctccgct agcagtgctg aagtaaacga tgatgatgat gaggatgatg
      241 gcgcagatca ctcccaagat gatcatcatc ttgaggtttt tccaccagta tttgcgcttg
      301 agcttggctg cacttgtttc aaactgggag gcccctgcct ggagggcatc tgcacggtca
      361 tccagctccg acaacttctg gtcccgctcc aggaccttgt ccacattcac cctcatgatg
      421 tccaccacct catccacctg ggcctgggtc tgctgcagtc tcctgttact agtaaggttt
      481 ggaggaggtg caggggggcc accctcgccg gccggggcgg caggcgggac ggtggcagcg
      541 gtagccgaca ttcctcctcc tgagcctcct cctgatccat c
//
