LOCUS       sequence-132201-         481 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV vector with CaMKIIa promoter, EGFP, and W3SL(shorten expression 
            cassette with relatively high expression to maximize expression of 
            larger genes) .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Choi JH, Yu NK, Baek GC, Bakes J, Seo D, Nam HJ, Baek SH, Lim CS, 
            Lee YS, Kaang BK
  TITLE     Optimization of AAV expression cassettes to improve packaging 
            capacity and transgene expression in neurons.
  JOURNAL   Mol Brain. 2014 Mar 11;7:17. doi: 10.1186/1756-6606-7-17.
  PUBMED    24618276
REFERENCE   2  (bases 1 to 481)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Brain."; date: "2014-03-11"; volume: "7:17. doi"; pages: " 
            10.1186/1756-6606-7-17"
COMMENT     Sequence Label: pAAV-CW3SL-EGFP
FEATURES             Location/Qualifiers
     source          1..481
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttgctcacca tggtggctag ctcgaatcaa gcttatcgat accgtctttt cacgctgccc
       61 ccagaactag gggccactcg cctgcccgtg ctcctgagtg caaacggaga accggcttga
      121 ctgacgagct tggggcttct gagcagggca ctgtggctgc tcacagcctc gcgagccttc
      181 gtcagcatcc aggtccccat ggcaaccacc tccgaaacgc cctgttcccg ttgccccggt
      241 aactgcctcc ccaacacctg cctgcctttc actttaaaac ctgctcctct ttgccctggc
      301 cttatataca caatactgca ggactgtgtg ggcccaggag caagtggacc ctgttccccc
      361 agggtgatgt actgaggggg ttggagaagg ggatgcgaac aaacttagtc cacaagtgtg
      421 cagcatctgc ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct
      481 c
//
