LOCUS       sequence-132212-         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Split GFP plasmid for visualization of Tau dimerization. Used 
            together with plasmid 71433.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Brunello CA, Yan X, Huttunen HJ
  TITLE     Internalized Tau sensitizes cells to stress by promoting formation 
            and stability of stress granules.
  JOURNAL   Sci Rep. 2016 Jul 27;6:30498. doi: 10.1038/srep30498.
  PUBMED    27460788
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-07-27"; volume: "6:30498. doi"; pages: " 
            10.1038/srep30498"
COMMENT     Sequence Label: pmGFP11C-­Tau
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      672..681
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             708..755
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
ORIGIN
        1 tggtccgtac tccacccaag tcgccgtctt ccgccaagag ccgcctgcag acagcccccg
       61 tgcccatgcc agacctgaag aatgtcaagt ccaagatcgg ctccactgag aacctgaagc
      121 accagccggg aggcgggaag gtgcagataa ttaataagaa gctggatctt agcaacgtcc
      181 agtccaagtg tggctcaaag gataatatca aacacgtccc gggaggcggc agtgtgcaaa
      241 tagtctacaa accagttgac ctgagcaagg tgacctccaa gtgtggctca ttaggcaaca
      301 tccatcataa accaggaggt ggccaggtgg aagtaaaatc tgagaagctt gacttcaagg
      361 acagagtcca gtcgaagatt gggtccctgg acaatatcac ccacgtccct ggcggaggaa
      421 ataaaaagat tgaaacccac aagctgacct tccgcgagaa cgccaaagcc aagacagacc
      481 acggggcgga gatcgtgtac aagtcgccag tggtgtctgg ggacacgtct ccacggcatc
      541 tcagcaatgt ctcctccacc ggcagcatcg acatggtaga ctcgccccag ctcgccacgc
      601 tagctgacga ggtgtctgcc tccctggcca agcagggttt ggcggtaccg cgggcccggg
      661 atccaccggt cgccaccatg ggcgacggcg gcagcggcgg cggcagccgg gaccacatgg
      721 tgctgcacga gtacgtgaac gccgccggca tcacataaag cggccgcgac tctagatcat
      781 aatcagccat accacatttg tagaggtttt acttgcttta aa
//
