LOCUS       sequence-132220-         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid encoding perch Cx35 with an internal 
            HaloTag between amino acids 285 and 286 in the C-terminus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Wang HY, Lin YP, Mitchell CK, Ram S, O'Brien J
  TITLE     Two-color fluorescent analysis of connexin 36 turnover: relationship
            to functional plasticity.
  JOURNAL   J Cell Sci. 2015 Nov 1;128(21):3888-97. doi: 10.1242/jcs.162586. 
            Epub 2015 Sep 10.
  PUBMED    26359298
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/jcs.162586"; journalName: "J Cell Sci"; date: "2015-11-1- 
            1"; volume: "128"; issue: "21"; pages: "3888-97"
COMMENT     Sequence Label: CMV-Cx35-Halo-C-in
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(124..144)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
ORIGIN
        1 tttttttttt ttacatattt tcacttcttc ctccccgttt gtgtgtgtgt atgtgtgtgt
       61 ttgttacaca taagcagagt cactggactg agtacggccg aaattaggca cactcatcct
      121 cgggctctga aagtacagat cctcagtggt tggctcgccg gaaatctcga gcgtcgacag
      181 ccagcgcgcg atctcgctgc cgatcaggtc cgggttgtct tcttgcagca gattcagacc
      241 cgggccgatg tccacagcct tgcagttagg caggcttttg gccaggcgag cggcttcggc
      301 cggtgggatc agaacgcctg gggtgcccca gaacagcagc ttcgggacag gggactggtg
      361 cagccagtcc atgtattctt cgaccagcgc gacgatgttc gctggctcac cggcgattgg
      421 cagctcgttt gggaagcgcc acagtggctc gcggtcaaca ggattcagga acggctcgcg
      481 gtaatggtcc atctcgactt cagtcagcgg gcggacgaca cccatcggca gcgtaccctc
      541 gataaaaacg ttctgatcga tgatcagctt gcggccgacg tcggtggtgc ggaaggcctg
      601 gaaggtctcg cgggcaaatt ctggccattc gtcccaggtc gggatagggc ggatgaactc
      661 cataaatgca atacctttga cgcgctctgg attgcgcttg gcccagtgga aacccagagc
      721 ggagccccag tcgtgaatga ccaggacgac ctcttccaga cccagggctt cgatgaaggc
      781 atccatgaag cggacgtggt cgt
//
