LOCUS       sequence-132223-         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Nishino J, Kim I, Chada K, Morrison SJ
  TITLE     Hmga2 promotes neural stem cell self-renewal in young but not old 
            mice by reducing p16Ink4a and p19Arf Expression.
  JOURNAL   Cell. 2008 Oct 17. 135(2):227-39.
  PUBMED    18957199
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2008 
            Oct 17. 135(2):227-39."
COMMENT     Sequence Label: HMGA2(3'UTR DEL)+GFP
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     400..416
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     401..417
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(459..492)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tggaattcga gctcggtacc ctcgaggcgg cgggaggcag gatgagcgca cgcggtgagg
       61 gcgccgggca gccgtccaca tcagcccagg gacaacctgc cgccccggtg ccacagaagc
      121 gaggacgcgg ccgacccagg aagcagcagc aagagccaac ctgtgagccc tctcctaaga
      181 gacccagagg aagacccaaa ggcagcaaaa acaagagccc ctctaaagca gcccagaaga
      241 aagcagagac cattggagaa aaacggccaa gaggcagacc taggaaatgg ccacaacaag
      301 tcgttcagaa gaagcctgct caggagactg aagagacatc ctcgcaagag tccgcagagg
      361 aggattagga attcctgcag cccgggggat cctctagagt cgaggtcgac ggtatcgata
      421 agctcgcttc acgagattcc agcaggtcga gggacctaat aacttcgtat agcatacatt
      481 atacgaagtt atattaaggg ttccaagctt aagcggccgc gtggataacc gtattaccgc
      541 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
      601 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
      661 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      721 gacgtcaatg ggtggagtat t
//
