LOCUS       sequence-132231-       12644 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used to label reporter mRNAs. Drosophila expression of the PCP 
            protein fused to EGFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12644)
  AUTHORS   Halstead JM, Lionnet T, Wilbertz JH, Wippich F, Ephrussi A, Singer 
            RH, Chao JA
  TITLE     Translation. An RNA biosensor for imaging the first round of 
            translation from single cells to living animals.
  JOURNAL   Science. 2015 Mar 20;347(6228):1367-671. doi: 
            10.1126/science.aaa3380.
  PUBMED    25792328
REFERENCE   2  (bases 1 to 12644)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2015-03-20- 20"; volume: "347"; 
            issue: "6228"; pages: "1367-671"
COMMENT     Sequence Label: pHsp83-NLS-HA-2xPCP-2xGFP
FEATURES             Location/Qualifiers
     source          1..12644
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(914..930)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(915..931)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             996..1016
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1020..1046
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             1020..1046
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1047..1070
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             join(1851..1853,1854..2564)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(1851..1853,1854..2564)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(2613..2615,2616..3329)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(2613..2615,2616..3326)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     misc_feature    join(4835..5036,5037..5067)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     complement(5606..5625)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5725..5747
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5785..5803)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5871..5975
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5976..6044,6045..6836)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6194..6213)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7007..7595
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7496..7515
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    join(7830..7860,7861..8415)
                     /label=P element 5' end
     primer_bind     12501..12519
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
ORIGIN
        1 atctttaacg gggaacttga agaagtgcat attggggttg cggctagaac ccaccggaca
       61 atcacgaaaa caacacttag tgccgcccat ttgtttaaat ataagcaaac aacttttatg
      121 ttattagtgg tggaagtgtt agcgtcagct ggtgatatcg atgggaggca tcgataacag
      181 aattgaccga aaccaaatga tcgatatgac acttcttaat taatgagaga ttttttactt
      241 gactgggctt gtagcaggtt ttgcacagaa gcaattattt tccggaatgt gaaatgtctg
      301 ctttttagct aattacaaca aaaactttcc aatttttgtt ccccaaaccc actcaagtga
      361 tttcaaattt taccgtccgc ttaaaatgga actagtttat tccagaggaa ccagcttgca
      421 ccaccaagtc tctgaaactc tggaaatatc gatagtctgg tggagaaaag tattcataaa
      481 tataaataaa aattaacagg tcataagctg atttgtttat tatttactgt taaaacaagt
      541 aaaataatat tgggaacaat taaattttcc attttcctaa ttacagtata agcctagtgg
      601 gcgttttgat atccaattgt aatgttttaa gcaatcccag tgggctttgc tcaatcgttc
      661 ggaccactta gacgaatttc caccaaactt agttcagtat aatttttgaa ttcgcccgca
      721 caggttgcgc acttttcgac cgtatcacaa cactgatcta ccctagtatt cacaggaagt
      781 tgcatccctg gcatccagaa gcctctagaa gtttctagag acttccagtt cgggtcgggt
      841 ttttctataa aagcagacgc gcggcgtttg ccggttcgag tcttgaaaaa aatttcgtat
      901 caagctagct tatcgatacc gtcgacctcg agggggggcc cggtacatcg aattcctgca
      961 gcccggggga tcgatccacc gcggccgcaa tgggcccaaa aaagaaaaga aaagttggct
     1021 acccctacga cgtgcccgac tacgccctgg aagttctgtt ccaggggccc ctgttaatta
     1081 acatgctagc ctccaaaacc atcgttcttt cggtcggcga ggctactcgc actctgactg
     1141 agatccagtc caccgcagac cgtcagatct tcgaagagaa ggtcgggcct ctggtgggtc
     1201 ggctgcgcct cacggcttcg ctccgtcaaa acggagccaa gaccgcgtat cgcgtcaacc
     1261 taaaactgga tcaggcggac gtcgttgatt ccggacttcc gaaagtgcgc tacactcagg
     1321 tatggtcgca cgacgtgaca atcgttgcga atagcaccga ggcctcgcgc aaatcgttgt
     1381 acgatttgac caagtccctc gtcgcgacct cgcaggtcga agatcttgtc gtcaaccttg
     1441 tgccgctggg ccgtgcggat ccgctagcct ccaaaaccat cgttctttcg gtcggcgagg
     1501 ctactcgcac tctgactgag atccagtcca ccgcagaccg tcagatcttc gaagagaagg
     1561 tcgggcctct ggtgggtcgg ctgcgcctca cggcttcgct ccgtcaaaac ggagccaaga
     1621 ccgcgtatcg cgtcaaccta aaactggatc aggcggacgt cgttgattcc ggacttccga
     1681 aagtgcgcta cactcaggta tggtcgcacg acgtgacaat cgttgcgaat agcaccgagg
     1741 cctcgcgcaa atcgttgtac gatttgacca agtccctcgt cgcgacctcg caggtcgaag
     1801 atcttgtcgt caaccttgtg ccgctgggcc gtgattctag acacgtgttg gtgagcaagg
     1861 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     1921 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     1981 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     2041 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     2101 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     2161 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     2221 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     2281 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     2341 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     2401 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     2461 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     2521 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagggcaca ggcagcaccg
     2581 gttcaggttc atcaggcaca gctagttcat tggtgagcaa gggcgaggag ctgttcaccg
     2641 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     2701 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     2761 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     2821 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     2881 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     2941 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     3001 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     3061 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     3121 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     3181 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     3241 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     3301 ctctcggcat ggacgagctg tacaagtaaa cgcgtggccg gtacctctag agtcgacctc
     3361 gaacgttaac gttaacgtaa cgttaactcg aggagcttga taacattata cctaaaccca
     3421 tggtcaagag taaacatttc tgcctttgaa gttgagaaca caattaagca tcccctggtt
     3481 aaacctgaca ttcatacttg ttaatagcgc cataaacata gcaccaattc gaagaatact
     3541 tgttaatagc gccataaaca tagcaccaat ttcgaagaaa ctcagttaaa agcaattagc
     3601 aattagcaat tagcaataac tctgctgact tcaaaacgag aagagttgca agtatttgta
     3661 aggcacagtt tatagaccac cgacggctca ttagggctcg tcatgtaact aagcgcggtg
     3721 aaacccaatt gaacatatag tggaattatt attatcaatg gggaagattt aaccctcagg
     3781 tagcaaagta atttaattgc aaatagagag tcctaagact aaataatata tttaaaaatc
     3841 tggccctttg accttgcttg tcaggtgcat ttgggttcaa tcgtaagttg cttctatata
     3901 aacactttcc ccatccccgc aataatgaag aataccgcag aataaagaga gatttgcaac
     3961 aaaaaataaa ggcattgcga aaacttttta tgggggatca ttacactcgg gcctacggtt
     4021 acaattccca gccacttaag cgacaagttt ggccaacaat ccatctaata gctaatagcg
     4081 caatcactgg taatcgcaag agtatatagg caatagaacc catggatttg accaaaggta
     4141 accgagacaa tggagaagca agaggatttc aaactgaaca cccacagtgc tgtgtactac
     4201 cactggcgcg tttgggagct cactggcctg atgcgtcctc cgggcgtttc aagcctgctt
     4261 tacgtggtat actccattac ggtcaacttg gtggtcaccg tgctgtttcc cttgagcttg
     4321 ctggccaggc tgctgttcac caccaacatg gccggattgt gcgagaacct gaccataact
     4381 attaccgata ttgtggccaa tttgaagttt gcgaatgtgt acatggtgag gaagcagctc
     4441 catgagattc gctctctcct aaggctcatg gacgctagag cccggctggt gggcgatccc
     4501 gaggagattt ctgccttgag gaaggaagtg aatatcgcac agggcacttt ccgcaccttt
     4561 gccagtattt tcgtatttgg cactactttg agttgcgtcc gcgtggtcgt tcgcccggat
     4621 cgagagctcc tgtatccggc ctggttcggc gttgactgga tgcactccac cagaaactat
     4681 gtgctcatca atatctacca gctcttcggc ttgatagtgc aggctataca gaactgcgct
     4741 agtgactcct atccgcctgc gtttctctgc ctgctcacgg gtcatatgcg tgctttggag
     4801 ctgagggtgc ggcggattgg ctgcagccaa gctttgcgta ctcgcaaatt attaaaaata
     4861 aaactttaaa aataatttcg tctaattaat attatgagtt aattcaaacc ccacggacat
     4921 gctaagggtt aatcaacaat catatcgctg tctcactcag actcaatacg acactcagaa
     4981 tactattcct ttcactcgca cttattgcaa gcatacgtta agtggatgtc tcttgccgac
     5041 gggaccacct tatgttattt catcatggtc tggccattct catcgtgagc ttccgggtgc
     5101 tcgcatatct ggctctaaga cttcgggccc gacgcaagga gtagccgaca tatatccgaa
     5161 ataactgctt gttttttttt tttaccatta ttaccatcgt gtttactgtt tattgccccc
     5221 tcaaaaagct aatgtaatta tatttgtgcc aataaaaaca agatatgacc tatagaatac
     5281 aagtatttcc ccttcgaaca tccccacaag tagactttgg atttgtcttc taaccaaaag
     5341 acttacacac ctgcatacct tacatcaaaa actcgtttat cgctacataa aacaccggga
     5401 tatatttttt atatacatac ttttcaaatc gcgcgccctc ttcataattc acctccacca
     5461 caccacgttt cgtagttgct ctttcgctgt ctcccacccg ctctccgcaa cacattcacc
     5521 ttttgttcga cgaccttgga gcgactgtcg ttagttccgc gcgattcggg tgcggtattt
     5581 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     5641 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     5701 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     5761 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     5821 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     5881 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     5941 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     6001 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     6061 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     6121 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     6181 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     6241 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     6301 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     6361 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     6421 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     6481 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     6541 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     6601 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     6661 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     6721 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     6781 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     6841 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     6901 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     6961 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     7021 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     7081 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     7141 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     7201 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     7261 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     7321 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     7381 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     7441 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     7501 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     7561 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cttcttcttg aactcgggct
     7621 cggtgccagt atacctcaaa tggttgtcgt acctctcatg gttccgttac gccaacgagg
     7681 gtctgctgat taaccaatgg gcggacgtgg agccgggcga aattagctgc acatcgtcga
     7741 acaccacgtg ccccagttcg ggcaaggtca tcctggagac gcttaacttc tccgccgccg
     7801 atctgccgct ggactacgtg ggtctggccc atgatgaaat aacataaggt ggtcccgtcg
     7861 atagccgaag cttaccgaag tatacactta aattcagtgc acgtttgctt gttgagagga
     7921 aaggttgtgt gcggacgaat ttttttttga aaacattaac ccttacgtgg aataaaaaaa
     7981 aatgaaatat tgcaaatttt gctgcaaagc tgtgactgga gtaaaattaa ttcacgtgcc
     8041 gaagtgtgct attaagagaa aattgtggga gcagagcctt gggtgcagcc ttggtgaaaa
     8101 ctcccaaatt tgtgataccc actttaatga ttcgcagtgg aaggctgcac ctgcaaaagg
     8161 tcagacattt aaaaggaggc gactcaacgc agatgccgta cctagtaaag tgatagagcc
     8221 tgaaccagaa aagataaaag aaggctatac cagtgggagt acacaaacag agtaagtttg
     8281 aatagtaaaa aaaatcattt atgtaaacaa taacgtgact gtgcgttagg tcctgttcat
     8341 tgtttaatga aaataagagc ttgagggaaa aaattcgtac tttggagtac gaaatgcgtc
     8401 gtttagagca gcagccgaat taattctagt tccagtgaaa tccaagcatt ttctaaatta
     8461 aatgtattct tattattata gttgttattt ttgatatata taaacaacac tattatgccc
     8521 accatttttt tgagatgcat ctacacaagg aacaaacact ggatgtcact ttcagttcaa
     8581 attgtaacgc taatcactcc gaacaggtca caaaaaatta ccttaaaaag tcataatatt
     8641 aaattagaat aaatatagct gtgagggaaa tatatacaaa tatattggag caaataaatt
     8701 gtacatacaa atatttatta ctaatttcta ttgagacgaa atgaaccact cggaaccatt
     8761 tgagcgaacc gaatcgcgcg gaactaacga cagtcgctcc aaggtcgtcg aacaaaaggt
     8821 gaatgtgttg cggagagcgg gtgggagaca gcgaaagagc aactacgaaa cgtggtgtgg
     8881 tggaggtgaa ttatgaagag ggcgcgcgat ttgaaaagta tgtatataaa aaatatatcc
     8941 cggtgtttta tgtagcgata aacgagtttt tgatgtaagg tatgcaggtg tgtaagtctt
     9001 ttggttagaa gacaaatcca aagtctactt gtggggatgt tcgaagggga aatacttgta
     9061 ttctataggt catatcttgt ttttattggc acaaatataa ttacattagc tttttgaggg
     9121 ggcaataaac agtaaacacg atggtaataa tggtaaaaaa aaaaaacaag cagttatttc
     9181 ggatatatgt cggctactcc ttgcgtcggg cccgaagtct tagagccaga tatgcgagca
     9241 cccggaagct cacgatgaga atggccagac ccacgtagtc cagcggcaga tcggcggcgg
     9301 agaagttaag cgtctccagg atgaccttgc ccgaactggg gcacgtggtg ttcgacgatg
     9361 tgcagctaat ttcgcccggc tccacgtccg cccattggtt aatcagcaga ccctcgttgg
     9421 cgtaacggaa ccatgagagg tacgacaacc atttgaggta tactggcacc gagcccgagt
     9481 tcaagaagaa gccgccaaag agcaggaatg gtatgataac cggcggaccc acagacagcg
     9541 ccatcgaggt cgaggagctg gcgcaggata ttagatatcc gaaggacgtt gacacattgg
     9601 ccaccagagt gaccagcgcc aggcagttga agaagtgcag cactccggcc cgcagtccga
     9661 tcatcggata ggcaatcgcc gtgaagacca gtggcactgt gagaaaaagc ggcaattcgg
     9721 caatcgtttt gcccagaaag tatgtgtcac agcgataaag tcgacttcgg gcctccctca
     9781 taaaaactgg cagctctgag gtgaacacct aaatcgaatc gattcattag aaagttagta
     9841 aattattgaa atgcaaatgt attctaaaca tgacttacat ttatcgtggc aaagacgttt
     9901 tgaaaggtca tgttggtcag gaagaggaag atggctccgt tgatattcat cacacccact
     9961 tgcgtgagtt gttggcccaa aaagatgagg ccaatcaaga tggcaaccat ctgcaaatta
    10021 aaatgttact cgcatctcat taatattcgc gagttaaatg aaatttattt atcttctgca
    10081 aaactataaa ctatacatct cattgaaaaa aactaagaag ggtgtggaat caggcaattc
    10141 tatctaaaat ctagcgaatt tgtttccaag aattgtaagc gttatatcat ttgtttccac
    10201 tggaaccact caccgttgtc tgaataagtc gcacttttac gaggagtggt tccttgagca
    10261 ccgacagcca ggatcgccac aggaccgccc ggaactgcat gaaccaggtg gccttgtagg
    10321 tgtacccatt ctccggctgc tccagtggct tctccagatt tttggtggcc aacaactgct
    10381 ccatatcccg ggctactttg ctaatggcaa aattgtcgca tatcttggcg atccgatcac
    10441 gggactcgat ctcccgtccg ggcacaacgg ccaacacctg tacgtaaaag tccgccggat
    10501 tgtagttggt aggacactgg gcacccacgc tggataggag ttgagatgcc cttaataaac
    10561 acatcgaact cactaggaaa agaagtcgac ggcttcgctg ggagtgccca agaaagctac
    10621 cctgccctcg gccatcagaa ggatcttgtc aaagagctca aacagctcgg aagacggctg
    10681 atgaatggtc aggatgacgg tcttgccctt ctgcgacagc ttcttcagca cctggacgac
    10741 gctgtgggcg gtaaaggagt ccagtccgga ggtgggctca tcgcagatca gaagcggcgg
    10801 atcggttaga gcctcggagg cgaatgccag acgcttcctt tctccgccgg acagaccttt
    10861 caccctgccg ggcacaccga tgatcgtgtg ctgacatttg ctgagcgaaa gctcctggat
    10921 cacctgatcc acgcgggcca ctcgctgccg ataggtcaga tgtcgtggca tccgcaccat
    10981 ggcttggaaa atcaggtgtt ccctggccgt tagggagccg ataaagaggt catcctgctg
    11041 gacataggcg cacctggcct gcatctcctt ggcgtccaca ggttggccat tgagcagtcg
    11101 catcccggat ggcgatactt ggatgccctg cggcgatcga aaggcaaggg cattcagcag
    11161 ggtcgtcttt ccggcaccgg aactgcccat cacggccaaa agttcgcccg gataggccac
    11221 gccgcaaact gagtttcaaa ttggtaattg gaccctttat taagatttca cacagatcag
    11281 ccgactgcga atagaaactc accgttcttg agcaaatgtt tcctgggcgc cggtatgtgt
    11341 cgctcgttgc agaatagtcc gcgtgtccgg ttgaccagct gccgccatcc ggagcccggc
    11401 tgattgaccg ccccaaagat gtccatattg tgccaggcat aggtgaggtt ctcggctagt
    11461 tggccgctcc ctgaaccgga gtcctccggc ggactgggtg gccggagcgt gccgtagttt
    11521 ttggcctgcc cgaagccctg gttaatgcag ctctgcgaag ccgctccgct gtcaccctgc
    11581 aatgataggg gatctcaaat atcaactaca agcgttatgc tcatctaacc ccgaacaaaa
    11641 agtaccccga agtatcctac gaagtaggtt tatactttta tttatttttt gtgcatctag
    11701 gatcagctta aaatatctgg ttgttatatt ttttgtaaaa aagaatatag tcgaaaatga
    11761 atgcctttag atgtcttgat catgatatga tctcaaaaat tgtcttatat agcgagaaca
    11821 gctaccagaa taatctgttt cgtgtcacta tttgtttgtg caattgcggt ttgggatttt
    11881 tgtgggtcgc agttctcacg ccgcagacaa tttgatgttg caatcgcagt tcctatagat
    11941 caagtgaact taagatgtat gcacatgtac tactcacatt gttcagatgc tcggcagatg
    12001 ggtgtttgct gcctccgcga attaatagct cctgatcctc ttggcccatt gccgggattt
    12061 ttcacacttt cccctgctta cccacccaaa accaatcacc accccaatca ctcaaaaaac
    12121 aaacaaaaat aagaagcgag aggagttttg gcacagcact ttgtgtttaa ttgatggcgt
    12181 aaaccgcttg gagcttcgtc acgaaaccgc tgacaaaatg caactgaagg cggacattga
    12241 cgctacgtaa cgctacaaac ggtggcgaaa gagatagcgg acgcagcggc gaaagagacg
    12301 gcgatatttc tgtggacaga gaaggaggca aacagcgctg actttgagtg gaatgtcatt
    12361 ttgagtgaga ggtaatcgaa agaacctggt acatcaaata cccttggatc gaagtaaatt
    12421 taaaactgat cagataagtt caatgatatc cagtgcagta aaaaaaaaaa atgttttttt
    12481 tatctacttt ccgcaaaaat gggttttatt aacttacata catactagaa ttggccgctc
    12541 tagccccccc tcgaatgttc tctctcttct cttctctctc tctttctcga atgttctctc
    12601 tcttctcttc tctctctctt tctcgaggtc atcaagctta ggag
//
