LOCUS       sequence-132232-        9744 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector of luciferase-eGFP fusion gene driven by FerH 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9744)
  AUTHORS   Day CP, Carter J, Bonomi C, Esposito D, Crise B, Ortiz-Conde B, 
            Hollingshead M, Merlino G
  TITLE     Lentivirus-mediated bifunctional cell labeling for in vivo melanoma 
            study.
  JOURNAL   Pigment Cell Melanoma Res. 2009 Jun;22(3):283-95. doi: 
            10.1111/j.1755-148X.2009.00545.x. Epub 2009 Jan 19.
  PUBMED    19175523
REFERENCE   2  (bases 1 to 9744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1755-148X.2009.00545.x"; journalName: "Pigment Cell 
            Melanoma Res"; date: "2009-06"; volume: "22"; issue: "3"; pages: 
            "283-95"
COMMENT     Sequence Label: pFUGW-FerH-ffLuc2-eGFP
FEATURES             Location/Qualifiers
     source          1..9744
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1686..1919
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2104..2148
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2446..2563
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2642..2662
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        2708..2899
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2731..2751)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     intron          3106..4053
                     /label=mEF-1-alpha intron
                     /note="intron upstream of the start codon of mouse 
                     EF-1-alpha"
     protein_bind    4085..4109
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4112..5761
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     regulatory      5777..5786
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5783..5785,5786..5788,5789..6502)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5783..5785,5786..5788,5789..6499)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5828..5849)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6089..6108)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6436..6457
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(6504..6528)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     6542..6558
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    6633..7221
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6686..6706)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7104..7115)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7224..7240)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             7750..7930
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(7992..8580)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8072..8091)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8751..9542,9543..9611))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9374..9393
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9612..9716)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg gccgcgctga tcttcagacc tggaggagga
     1561 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     1621 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     1681 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     1741 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     1801 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1861 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1921 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1981 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     2041 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     2101 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     2161 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     2221 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     2281 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     2341 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
     2401 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
     2461 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2521 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2581 cagcagagat ccagtttggt tagtaccggg cccgctctag attgaattca agggatcatc
     2641 acaactttgt atagaaaagt tggcggcctg aaataacctc tgaaagagga acttggttag
     2701 gtaccttctg aggctgaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt
     2761 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     2821 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     2881 agtcagcaac catagtccca ctagttccgc cagagcgcgc gagggcctcc agcggccgcc
     2941 cctcccccac agcaggggcg gggtcccgcg cccaccggaa ggagcgggct cggggcgggc
     3001 ggcgctgatt ggccggggcg ggcctgacgc cgacgcggct ataagagacc acaagcgacc
     3061 cgcagggcca gacgttcttc gccgaagctt gccgtcagaa cgcaggtgag gggcgggtgt
     3121 ggcttccgcg ggccgccgag ctggaggtcc tgctccgagc gggccgggcc ccgctgtcgt
     3181 cggcggggat tagctgcgag cattcccgct tcgagttgcg ggcggcgcgg gaggcagagt
     3241 gcgaggccta gcggcaaccc cgtagcctcg cctcgtgtcc ggcttgaggc ctagcgtggt
     3301 gtccgcgccg ccgccgcgtg ctactccggc cgcactctgg tctttttttt ttttgttgtt
     3361 gttgccctgc tgccttcgat tgccgttcag caataggggc taacaaaggg agggtgcggg
     3421 gcttgctcgc ccggagcccg gagaggtcat ggttggggag gaatggaggg acaggagtgg
     3481 cggctggggc ccgcccgcct tcggagcaca tgtccgacgc cacctggatg gggcgaggcc
     3541 tggggttttt cccgaagcaa ccaggctggg gttagcgtgc cgaggccatg tggccccagc
     3601 acccggcacg atctggcttg gcggcgccgc gttgccctgc ctccctaact agggtgaggc
     3661 catcccgtcc ggcaccagtt gcgtgcgtgg aaagatggcc gctcccgggc cctgttgcaa
     3721 ggagctcaaa atggaggacg cggcagcccg gtggagcggg cgggtgagtc acccacacaa
     3781 aggaagaggg cctggtccct caccggctgc tgcttcctgt gaccccgtgg tcctatcggc
     3841 cgcaatagtc acctcgggct tttgagcacg gctagtcgcg gcggggggag gggatgtaat
     3901 ggcgttggag tttgttcaca tttggtgggt ggagactagt caggccagcc tggcgctgga
     3961 agtcattttt ggaatttgtc cccttgagtt ttgagcggag ctaattctcg ggcttcttag
     4021 cggttcaaag gtatctttta aacccttttt taggtgttgt gaaaaccacc gctaattcaa
     4081 agcaacaact ttgtacaaaa aagttggcac catggaagat gccaaaaaca ttaagaaggg
     4141 cccagcgcca ttctacccac tcgaagacgg gaccgccggc gagcagctgc acaaagccat
     4201 gaagcgctac gccctggtgc ccggcaccat cgcctttacc gacgcacata tcgaggtgga
     4261 cattacctac gccgagtact tcgagatgag cgttcggctg gcagaagcta tgaagcgcta
     4321 tgggctgaat acaaaccatc ggatcgtggt gtgcagcgag aatagcttgc agttcttcat
     4381 gcccgtgttg ggtgccctgt tcatcggtgt ggctgtggcc ccagctaacg acatctacaa
     4441 cgagcgcgag ctgctgaaca gcatgggcat cagccagccc accgtcgtat tcgtgagcaa
     4501 gaaagggctg caaaagatcc tcaacgtgca aaagaagcta ccgatcatac aaaagatcat
     4561 catcatggat agcaagaccg actaccaggg cttccaaagc atgtacacct tcgtgacttc
     4621 ccatttgcca cccggcttca acgagtacga cttcgtgccc gagagcttcg accgggacaa
     4681 aaccatcgcc ctgatcatga acagtagtgg cagtaccgga ttgcccaagg gcgtagccct
     4741 accgcaccgc accgcttgtg tccgattcag tcatgcccgc gaccccatct tcggcaacca
     4801 gatcatcccc gacaccgcta tcctcagcgt ggtgccattt caccacggct tcggcatgtt
     4861 caccacgctg ggctacttga tctgcggctt tcgggtcgtg ctcatgtacc gcttcgagga
     4921 ggagctattc ttgcgcagct tgcaagacta taagattcaa tctgccctgc tggtgcccac
     4981 actatttagc ttcttcgcta agagcactct catcgacaag tacgacctaa gcaacttgca
     5041 cgagatcgcc agcggcgggg cgccgctcag caaggaggta ggtgaggccg tggccaaacg
     5101 cttccaccta ccaggcatcc gccagggcta cggcctgaca gaaacaacca gcgccattct
     5161 gatcaccccc gaaggggacg acaagcctgg cgcagtaggc aaggtggtgc ccttcttcga
     5221 ggctaaggtg gtggacttgg acaccggtaa gacactgggt gtgaaccagc gcggcgagct
     5281 gtgcgtccgt ggccccatga tcatgagcgg ctacgttaac aaccccgagg ctacaaacgc
     5341 tctcatcgac aaggacggct ggctgcacag cggcgacatc gcctactggg acgaggacga
     5401 gcacttcttc atcgtggacc ggctgaagag cctgatcaaa tacaagggct accaggtagc
     5461 cccagccgaa ctggagagca tcctgctgca acaccccaac atcttcgacg ccggggtcgc
     5521 cggcctgccc gacgacgatg ccggcgagct gcccgccgca gtcgtcgtgc tggaacacgg
     5581 taaaaccatg accgagaagg agatcgtgga ctatgtggcc agccaggtta caaccgccaa
     5641 gaagctgcgc ggtggtgttg tgttcgtgga cgaggtgcct aaaggactga ccggcaagtt
     5701 ggacgcccgc aagatccgcg agattctcat taaggccaag aagggcggca agatcgccgt
     5761 gcgggatcca ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
     5821 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     5881 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     5941 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     6001 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     6061 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     6121 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     6181 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     6241 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     6301 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     6361 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     6421 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     6481 catggacgag ctgtacaagt agtaaccaac tttcttgtac aaagttgtga tgatccaatc
     6541 tcgaggtcga cggtatcgat aagctcgctt cacgagatca tgtttaaggg ttccggttcc
     6601 actaggtaca attcgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg
     6661 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     6721 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     6781 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     6841 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     6901 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     6961 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     7021 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     7081 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     7141 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     7201 ccctttgggc cgcctccccg catcgatacc gtcgacctcg atcgagacct agaaaaacat
     7261 ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca
     7321 caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact
     7381 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     7441 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
     7501 ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga
     7561 tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag
     7621 aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta
     7681 ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg
     7741 gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     7801 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     7861 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     7921 atctctagca gcatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     7981 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     8041 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     8101 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     8161 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     8221 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     8281 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     8341 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     8401 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     8461 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     8521 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     8581 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     8641 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     8701 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     8761 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     8821 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     8881 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     8941 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     9001 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     9061 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     9121 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     9181 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     9241 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     9301 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     9361 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     9421 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     9481 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     9541 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     9601 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     9661 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     9721 tttccccgaa aagtgccacc tgac
//
