LOCUS       sequence-132250-         743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of GFP-BRCA1 1364-1437.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Orthwein A, Noordermeer SM, Wilson MD, Landry S, Enchev RI, Sherker 
            A, Munro M, Pinder J, Salsman J, Dellaire G, Xia B, Peter M, 
            Durocher D
  TITLE     A mechanism for the suppression of homologous recombination in G1 
            cells.
  JOURNAL   Nature. 2015 Dec 9. doi: 10.1038/nature16142.
  PUBMED    26649820
REFERENCE   2  (bases 1 to 743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Dec 9. doi: 10.1038/nature16142."
COMMENT     Sequence Label: pDEST-FRT/T0-GFP-BRCA1 1364-1437
FEATURES             Location/Qualifiers
     source          1..743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(312..329)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    318..542
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(675..694)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aaagcggaaa gtgaacttag gtgaagcagc atctgggtgt gagagtgaaa caagcgtctc
       61 tgaagactgc tcagggctat cctctcagag tgacatttta accactcagc agagggatac
      121 catgcaacat aacctgataa agctccagca ggaaatggct gaactagaag ctgtgttaga
      181 acagcatggg agccagcctt ctaacagcta ccctagtata atcagtgact cttcttaacc
      241 ggaattctgc agatatccat cacactggcg gccgctcgag tctagagggc ccgtttaaac
      301 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
      361 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
      421 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
      481 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
      541 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
      601 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
      661 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
      721 tccccgtcaa gctctaaatc ggg
//
