LOCUS       sequence-132287-         680 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor plasmid - kanamycin resistant donor cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Tas H, Nguyen CT, Patel R, Kim NH, Kuhlman TE
  TITLE     An Integrated System for Precise Genome Modification in Escherichia 
            coli.
  JOURNAL   PLoS One. 2015 Sep 2;10(9):e0136963. doi: 
            10.1371/journal.pone.0136963. eCollection 2015.
  PUBMED    26332675
REFERENCE   2  (bases 1 to 680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-2"; volume: "10(9):e0136963. doi"; pages: " 
            10.1371/journal.pone.0136963. eCollection 2015"
COMMENT     Sequence Label: pTKDP-neo
FEATURES             Location/Qualifiers
     source          1..680
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        12..59
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     primer_bind     complement(141..160)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 acctgcagcc tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca
       61 aggtgaggaa ctaaaccatg ggatcggcca ttgaacaaga tggattgcac gcaggttctc
      121 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
      181 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
      241 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
      301 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
      361 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
      421 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
      481 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
      541 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
      601 ccaggctcaa ggcgcgcatg cccgacggcg atgatctcgt cgtgacccat ggcgatgcct
      661 gcttgccgaa tatcatggtg
//
