LOCUS       sequence-132289-         613 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor plasmid - kanamycin resistant donor cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Tas H, Nguyen CT, Patel R, Kim NH, Kuhlman TE
  TITLE     An Integrated System for Precise Genome Modification in Escherichia 
            coli.
  JOURNAL   PLoS One. 2015 Sep 2;10(9):e0136963. doi: 
            10.1371/journal.pone.0136963. eCollection 2015.
  PUBMED    26332675
REFERENCE   2  (bases 1 to 613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-2"; volume: "10(9):e0136963. doi"; pages: " 
            10.1371/journal.pone.0136963. eCollection 2015"
COMMENT     Sequence Label: pTKDP-neo
FEATURES             Location/Qualifiers
     source          1..613
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(44..77)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(99..115)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(100..116)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        202..306
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(525..544)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cagccaagct agcttggctg gacgtaaact cctcttcaga cctgaagttc ctatactttc
       61 tagagaatag gaacttcgga attcgatatc aagcttatcg ataccgtcga cctcgagggg
      121 gggcccggta ccttcaccgt ttggaccttg gggccgtaat taccctgtta tccctaaggt
      181 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
      241 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
      301 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
      361 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcanttg
      421 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
      481 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
      541 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
      601 gacttggttg agt
//
