LOCUS       sequence-132294-         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway ORF clone of human NRAS [NM_002524.4] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E146 Hs.NRAS-nostop
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    85..184
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(752..851)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ggtgtgcgtc acccggcaac cttgggtagc agcgaagtcg aggcatttct gtcctggctg
       61 gtctagagga tctaagcttg catgcaaata atgattttat tttgactgat agtgacctgt
      121 tcgttgcaac aaattgataa gcaatgcttt cctataatgc caactttgta caagaaagtt
      181 gacatcacca cacatggcaa tcccatacaa ccctgagtcc catcatcact gctgttgagt
      241 tttttcattc ggtactggcg tatttctctt accagtgtgt aaaaagcatc ttcaacaccc
      301 tgtctggtct tggctgaggt ttcaatgaat ggaatcccgt aactcttggc cagttcgtgg
      361 gcttgttttg tatcaactgt ccttgttggc aaatcacact tgtttcccac tagcaccata
      421 ggtacatcat ccgagtcttt tactcgctta atctgctccc tgtagaggtt aatatccgca
      481 aatgacttgc tattattgat ggcaaataca cagaggaagc cttcgcctgt cctcatgtat
      541 tggtctctca tggcactgta ctcttcttgt ccagctgtat ccagtatgtc caacaaacag
      601 gtttcaccat ctataaccac ttgttttctg taagaatcct ctatggtggg atcatattca
      661 tctacaaagt ggttctggat tagctggatt gtcagtgcgc ttttcccaac accacctgct
      721 ccaaccacca ccagtttgta ctcagtcatg gtgccaactt ttttgtacaa agttggcatt
      781 ataaaaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa
      841 atcattattt cggatcctc
//
