LOCUS       sequence-132297-         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytosolic FRET, Excitation: , Emission: .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Davidson Lab
  TITLE     FRET Indicators - Live and Fixed Cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: mPAGFP-mCherry-FRET-10
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(401..420)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     670..688
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
       61 taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatctaga gtcgcggccg
      121 ctttacttgt acagctcgtc catgccgccg gtggagtggc ggccctcggc gcgttcgtac
      181 tgttccacga tggtgtagtc ctcgttgtgg gaggtgatgt ccaacttgat gttgacgttg
      241 taggcgccgg gcagctgcac gggcttcttg gccttgtagg tggtcttgac ctcagcgtcg
      301 tagtggccgc cgtccttcag cttcagcctc tgcttgatct cgcccttcag ggcgccgtcc
      361 tcggggtaca tccgctcgga ggaggcctcc cagcccatgg tcttcttctg cattacgggg
      421 ccgtcggagg ggaagttggt gccgcgcagc ttcaccttgt agatgaactc gccgtcctgc
      481 agggaggagt cctgggtcac ggtcaccacg ccgccgtcct cgaagttcat cacgcgctcc
      541 cacttgaagc cctcggggaa ggacagcttc aagtagtcgg ggatgtcggc ggggtgcttc
      601 acgtaggcct tggagccgta catgaactga ggggacagga tgtcccaggc gaagggcagg
      661 gggccaccct tggtcacctt cagcttggcg gtctgggtgc cctcgtaggg gcggccctcg
      721 ccctcgccct cgatctcgaa ctcgtggccg ttcacggagc cctccatgtg caccttgaag
      781 cgcatgaact ccttgatgat ggccatgtta tcctcctcgc c
//
