LOCUS       sequence-132308-        1238 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 490 / 553, Emission: 507 / 
            573.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1238)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Dendra2-FTR-952
FEATURES             Location/Qualifiers
     source          1..1238
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      211..220
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(217..219,220..222,223..936)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(262..283)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(523..542)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     870..891
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agctttagtg aaccgtcaga tccgctagcg ctaccggact cagatctcga gctcaagctt
       61 atgggcatgg cgcgaccacg gggcgcgggg ccctgcagcc ccgggttgga gcgggctccg
      121 cgccggagcg tcggggagct gcgcctgctc ttcgaagcgc gctgcgccgc agtcgccgcc
      181 gcagcggccg cagggtcgga tccaccggtc gccaccatgg tgagcaaggg cgaggagctg
      241 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
      301 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
      361 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
      421 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
      481 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
      541 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
      601 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
      661 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
      721 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
      781 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccaagctg
      841 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
      901 gggatcactc tcggcatgga cgagctgtac aagtaaagcg gccgcgactc tagatcataa
      961 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccac acctccccct
     1021 gaactgaaac ataaatgatg catgtgtgta actgtttatg cagctatatg tacaataagc
     1081 atagcatcac aaattcacaa ataagcattt ttttcactgc atctagctgt gcgtcgactc
     1141 catcatgtat tcttaaggcc taaatgtagc gttaatattg tcagtccgtc agttctgtat
     1201 cagcctaatt taaccaatag ggcgcgaatc cgcgccat
//
