LOCUS       sequence-132311-        2051 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 516, Emission: 529.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2051)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCitrine-MAP4-C-10
FEATURES             Location/Qualifiers
     source          1..2051
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(35..37,38..40,41..751)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(80..101)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(341..360)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     688..709
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt
       61 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      121 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      181 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttcg gctacggcct
      241 gatgtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      301 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      361 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      421 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
      481 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
      541 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
      601 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccaagctgag
      661 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
      721 gatcactctc ggcatggacg agctgtacaa gtccggactc agatctggtg gctccgcagg
      781 atcccggcaa gaagaagcaa aggctgctgt aggtgtgact ggaaatgaca tcactacccc
      841 gccaaacaag gagccaccac caagcccaga aaagaaagca aagcctttgg ccaccactca
      901 acctgcaaag acttcaacat cgaaagccaa aacacagccc acttctctcc ctaagcaacc
      961 agctcccacc acctctggtg ggttgaataa aaaacccatg agcctcgcct caggctcagt
     1021 gccagctgcc ccacacaaac gccctgctgc tgccactgct actgccaggc cttccaccct
     1081 acctgccaga gacgtgaagc caaagccaat tacagaagct aaggttgccg aaaagcggac
     1141 ctctccatcc aagccttcat ctgccccagc cctcaaacct ggacctaaaa ccaccccaac
     1201 cgtttcaaaa gccacatctc cctcaactct tgtttccact ggaccaagta gtagaagtcc
     1261 agctacaact ctgcctaaga ggccaaccag catcaagact gaggggaaac ctgctgatgt
     1321 caaaaggatg actgctaagt ctgcctcagc tgacttgagt cgctcaaaga ccacctctgc
     1381 cagttctgtg aagagaaaca ccactcccac tggggcagca cccccagcag ggatgacttc
     1441 cactcgagtc aagcccatgt ctgcacctag ccgctcttct ggggctcttt ctgtggacaa
     1501 gaagcccact tccactaagc ctagctcctc tgctcccagg gtgagccgcc tggccacaac
     1561 tgtttctgcc cctgacctga agagtgttcg ctccaaggtc ggctctacag aaaacatcaa
     1621 acaccagcct ggaggaggcc gggccaaggt agagaaaaaa acagaggcag ctaccacagc
     1681 tgggaagcct gaacctaatg cagtcactaa agcagccggc tccattgcga gtgcacagaa
     1741 accgcctgct gggaaagtcc agatagtatc caaaaaagtg agctacagtc atattcaatc
     1801 caagtgtgtt tccaaggaca atattaagca tgtccctgga tgtggcaatg ttcagattca
     1861 gaacaagaaa gtggacatat ccaaggtctc ctccaagtgt gggtccaaag ctaatatcaa
     1921 gcacaagcct ggtggaggag atgtcaagat tgaaagtcag aagttgaact tcaaggagaa
     1981 ggcccaagcc aaagtgggat agggatccac cggatctaga taactgatca taatcagcca
     2041 taccacattt g
//
