LOCUS       sequence-132312-         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-Sec61-N-18
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..330
                     /codon_start=1
                     /gene="SEC61B"
                     /product="human Sec61-beta subunit of the translocon in the
                     endoplasmic reticulum"
                     /label=Sec61-beta
                     /translation="MPGPTPSGTNVGSSGRSPSKAVAARAAGSTVRQRKNASCGTRSAG
                     RTTSAGTGGMWRFYTEDSPGLKVGPVPVLVMSLLFIASVFMLHIWGKYTRS"
     regulatory      379..388
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(518..536)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gcagagctgg tttagtgaac cgtcagatcc gctagcgcca ccatgcctgg tccgaccccc
       61 agtggcacta acgtgggatc ctcagggcgc tctcccagca aagcagtggc cgcccgggcg
      121 gcgggatcca ctgtccggca gaggaaaaat gccagctgtg ggacaaggag tgcaggccgc
      181 acaacctcgg caggcaccgg ggggatgtgg cgattctaca cagaagattc acctgggctc
      241 aaagttggcc ctgttccagt attggttatg agtcttctgt tcatcgcttc tgtatttatg
      301 ttgcacattt ggggcaagta cactcgttcg ggacgaattc tgcagtcgac ggtaccgcgg
      361 gcccgggatc caccggtcgc caccatggtg agcaagggcg aggaggtcat caaagagttc
      421 atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc
      481 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc
      541 ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg ctccaaggcg
      601 tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc cgagggcttc
      661 aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc
      721 tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa cttcccc
//
