LOCUS       sequence-132323-         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 588, Emission: 635.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKate2.6-CytERM-N-17
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      215..224
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             221..919
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSKTFINHPQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPANGPVMQKKTLGWEASTETLYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPAKNLKMPGVYNVDRRLERIKEADNETYVEQHEVAVARY
                     STGGAGDGGK"
ORIGIN
        1 tgaaccgtca gatccgctag cgctaccgga ctcagatctg gtaccccttg cagcttccag
       61 ttgtcaggaa gagaaggctc ccatggatcc tgtggtggtg ctggggctct gtctctcctg
      121 tttgcttctc ctttcactct ggaaacagag ctatggggga gggaagcttc gaattctgca
      181 gtcgacggta ccgcgggccc gggatccacc ggtcgccacc atggtgagcg agctgattaa
      241 ggagaacatg cacatgaagc tgtacatgga gggcaccgtg aacaaccacc acttcaagtg
      301 cacatccgag ggcgaaggca agccctacga gggcacccag accatgagaa tcaaggtcgt
      361 cgagggcggc cctctcccct tcgccttcga catcctggct accagcttca tgtacggcag
      421 caaaaccttc atcaaccacc ctcagggcat ccccgacttc tttaagcagt ccttccctga
      481 gggcttcaca tgggagagag tcaccacata cgaagacggg ggcgtgctga ccgctaccca
      541 ggacaccagc ctccaggacg gctgcctcat ctacaacgtc aagattagag gggtgaactt
      601 cccagccaac ggccctgtga tgcagaagaa aacactcggc tgggaggcct ccaccgagac
      661 gctgtacccc gctgacggcg gcctggaagg cgcatgtgac atggccctga agctcgtggg
      721 cgggggccac ctgatctgca acttggagac cacatacaga tccaagaaac ccgctaagaa
      781 cctcaagatg cccggcgtct acaacgtgga caggagactg gaaagaatca aggaggccga
      841 caatgagacc tacgtcgagc agcacgaggt ggctgtggcc agatactcta ctggtggcgc
      901 tggtgatgga ggtaaatgag cggccgcgac tctagatcat aatcagccat accacatttg
      961 tagaggtttt acttgcttta aaaaacctcc cac
//
