LOCUS       sequence-132327-         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytosolic FRET, Excitation:  / 554, Emission:  / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Davidson Lab
  TITLE     FRET Indicators - Live and Fixed Cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: mVenus-tdTomato-FRET-10
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(75..77,78..80,81..791)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(120..141)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(381..400)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     728..749
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      816..825
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gagctggttt agtgaaccgt cagatccgct agcgctaccg gactcagatc tcgagctcaa
       61 gcttcgaatt caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
      121 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
      181 gcgatgccac ctacggcaag ctgaccctga agctgatctg caccaccggc aagctgcccg
      241 tgccctggcc caccctcgtg accaccctgg gctacggcct gcagtgcttc gcccgctacc
      301 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
      361 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
      421 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
      481 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcaccgccg
      541 acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggcg
      601 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
      661 tgcccgacaa ccactacctg agctaccagt ccaaactgag caaagacccc aacgagaagc
      721 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
      781 agctgtacaa gtccggactc agatccccac cggtcgccac catggtgagc aagggcgagg
      841 aggtcatcaa agagttcatg cgcttcaagg tgcgcatgga gggctccatg aacggcca
//
