LOCUS       sequence-132340-         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway ORF clone of human MRAS [NM_001085049.2] with stop codon 
            (for native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E135 Hs.MRAS
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    85..184
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(812..911)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(941..958)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(941..957)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggtgtgcgtc acccggcaac cttgggtagc agcgaagtcg aggcatttct gtcctggctg
       61 gtctagagga tctaagcttg catgcaaata atgattttat tttgactgat agtgacctgt
      121 tcgttgcaac aaattgataa gcaatgcttt cctataatgc caactttgta caagaaagtt
      181 gattacaaga tcacacattg cagtttgtgg gtgcctgtgg cccggtctcc ccgccatttg
      241 gttttcttct tcttcttctg gcttttttcc ggaatctgtt gcctaattac tctaacgagg
      301 tcatggaagg ctttgtcgac attgagaggt gggtccttgg cactggtttc tatgtacgga
      361 atattgtgtt tggtcgccat ttcttttcct tgctccctgg tgatcttcct caagtgcatc
      421 aaatcgacct tgttggccac gaggatcatc gggaatgact ccctgtcttt gacgcgcagg
      481 ataagctggt ggaagcggtc cacgtgctca aagctggcct tgtcagtgac ggagtagacg
      541 atgaggaagc catcccccgt gcgcatgtat tgctcccgca tggcgctgaa ttcctcctgc
      601 ccagctgtgt ccagaacgtc caagatggcc cattgattgt caatctccgt atgtttcagg
      661 taggagtctt caatggtggg gtcatagtca ggcacaaaga tcttctggaa aaactggatg
      721 gtgagggcac ttttgcccac acccccatcc cccaccacca ccagcttgta tgtggggagg
      781 ttgtcactgg ggacggcgct ggttgccatg gtgccaactt ttttgtacaa agttggcatt
      841 ataaaaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa
      901 atcattattt cggatcctcg gaccgagctc gaattttaag actggccgtc gttttacaac
//
