LOCUS       sequence-132347-         729 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tn7-based lux reporter for promoter cloning in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Glassing A, Lewis TA
  TITLE     An improved Tn7-lux reporter for broad host range, 
            chromosomally-integrated promoter fusions in Gram-negative bacteria.
  JOURNAL   J Microbiol Methods. 2015 Sep 1;118:75-77. doi: 
            10.1016/j.mimet.2015.08.016.
  PUBMED    26341612
REFERENCE   2  (bases 1 to 729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Microbiol
            Methods."; date: "2015-09-1"; volume: "118:75-77. doi"; pages: " 
            10.1016/j.mimet.2015.08.016"
COMMENT     Sequence Label: pAG4
FEATURES             Location/Qualifiers
     source          1..729
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ctgggtgtag cgtcgtaagc taatacgaaa attaaaaatg acaaaatagt ttggaactag
       61 atttcactta tctggttggc ctgcaaggcc ttcgcgaggt acccaattcc tttaatccct
      121 ttaattcctg gattttttct ccgaaatctg ggagcgtata aacatctgga ctcgtataaa
      181 tcagttgact ggtttctatg ccttctttgc cacatattat atgtatatca actaataccg
      241 tattattatc ataacctata gacatagttc cattgtattt tagcgcgttc tcaaattctt
      301 cttttgatgg atacgatgat ccatctccgt actttttcgt taacgatttg cttactggct
      361 tcacaagtct gccaatatta atctctaatc ttttaaattt cccatttaaa aaaaatgcca
      421 ttgcttcacg ctttttgtta gcaaacgaaa agtcagaaca tgaataaagt gatacttctt
      481 ctattcgaga atctccctca aatttttcaa aatcacataa cctcttcgaa agaaactctt
      541 cttctgtcat gccaaacttt gcatctttat acccatcaac tgccattgca ccaaagctaa
      601 tcattcccaa tgcaagcata actgccaatt ttttcatgtt atctcctttt gctttgagcc
      661 aataataaca atgagaacaa tgttaaacca accccataaa cctctatcaa gtacccaaat
      721 gtggatgtt
//
