LOCUS       sequence-132366-         718 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vectors for the salycilate cascade expression system 
            (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm- nasF
            attenuator-T7 SD sequence-MCSII, MCS M13 replication origin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Medina C, Camacho EM, Flores A, Mesa-Pereira B, Santero E
  TITLE     Improved expression systems for regulated expression in Salmonella 
            infecting eukaryotic cells.
  JOURNAL   PLoS One. 2011;6(8):e23055. doi: 10.1371/journal.pone.0023055. Epub 
            2011 Aug 1.
  PUBMED    21829692
REFERENCE   2  (bases 1 to 718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(8):e23055. doi"; pages: " 
            10.1371/journal.pone.0023055. Epub 2011 Aug 1"
COMMENT     Sequence Label: pMPO 61
FEATURES             Location/Qualifiers
     source          1..718
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..26
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7..25
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             100..122
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        complement(561..641)
                     /label=Pm promoter
                     /note="The bacterial Pm promoter is activated by XylS in 
                     the presence of benzoate or m-toluate (Marques et al., 
                     1999)."
ORIGIN
        1 cgcgcgtaat acgactcact atagggcgaa ttggcgataa gcttgtcgac ggatccgagc
       61 tcggtaccat ggatatctgc agttcttgcc atatgtatat ctccttctta aagttaaaca
      121 aaattatttc ccgggtgaac cgaattggga attcgcgcaa aaaaaaagcg cccggcggtg
      181 cttacgcacc gctggacgcc tttatccctg gacatacgcg ccgccattgg cgcgctgccc
      241 aaaacctttt attcactcga attcaggcat tgacgaaggc attcagctca ccatctttgt
      301 tgcgtgtgat gaatatcggc tgccggccca tctgcgtggt gtaatagtcg ttcttctcgg
      361 gaatctggct ctcgtgggcg agataaatcc agttgccctc aaagatgtgt ttcatctcta
      421 aatcgaacag ccgagggtcg gtgaacatct cgcgcttgca gcggtagatg ccctcgttct
      481 catcttcttc aacgagacta tctatatagt cgagccccag gtgcattgtc atggtcatga
      541 ctccattatt attgtttctg ttgcataaag cctaaggggt aggcctttct agagatagcc
      601 attttttgca ctcctgtatc cgcttcttgc aaggctggac ttatctctat caaattccct
      661 ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttcg caacgttc
//
