LOCUS       sequence-132367-        5744 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vectors for the salycilate cascade expression system 
            (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm- nasF
            attenuator-T7 SD sequence-MCSII, MCS M13 replication origin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Medina C, Camacho EM, Flores A, Mesa-Pereira B, Santero E
  TITLE     Improved expression systems for regulated expression in Salmonella 
            infecting eukaryotic cells.
  JOURNAL   PLoS One. 2011;6(8):e23055. doi: 10.1371/journal.pone.0023055. Epub 
            2011 Aug 1.
  PUBMED    21829692
REFERENCE   2  (bases 1 to 5744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(8):e23055. doi"; pages: " 
            10.1371/journal.pone.0023055. Epub 2011 Aug 1"
COMMENT     Sequence Label: pMPO 61
FEATURES             Location/Qualifiers
     source          1..5744
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      409..495
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        590..670
                     /label=Pm promoter
                     /note="The bacterial Pm promoter is activated by XylS in 
                     the presence of benzoate or m-toluate (Marques et al., 
                     1999)."
     RBS             1109..1131
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(1205..1224)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1206..1224)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1234..1251)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1234..1250)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1243..1265)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1392..1847
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1479..1498)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1689..1710
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1873..1977
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1978..2046,2047..2838)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2196..2215)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(3861..4811)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      4859..5081
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
ORIGIN
        1 tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct
       61 gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca
      121 agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct
      181 cgccgaaaat gacccagagc gctgccggca ccctgtccta cgagttgcat gataaagaag
      241 acagtcataa gtgcggcgac gaccaccgcg gtggcggccg cgcgaatttg cctggcggca
      301 gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg
      361 atggtagtgt ggggtctccc catgcgagag tagggaactg ccaggcatca aataaaacga
      421 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
      481 ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg
      541 tggcgggcag gacgcccgcc ataaactgcc agggaatttg atagagataa gtccagcctt
      601 gcaagaagcg gatacaggag tgcaaaaaat ggctatctct agaaaggcct accccttagg
      661 ctttatgcaa cagaaacaat aataatggag tcatgaccat gacaatgcac ctggggctcg
      721 actatataga tagtctcgtt gaagaagatg agaacgaggg catctaccgc tgcaagcgcg
      781 agatgttcac cgaccctcgg ctgttcgatt tagagatgaa acacatcttt gagggcaact
      841 ggatttatct cgcccacgag agccagattc ccgagaagaa cgactattac accacgcaga
      901 tgggccggca gccgatattc atcacacgca acaaagatgg tgagctgaat gccttcgtca
      961 atgcctgaat tcgagtgaat aaaaggtttt gggcagcgcg ccaatggcgg cgcgtatgtc
     1021 cagggataaa ggcgtccagc ggtgcgtaag caccgccggg cgcttttttt ttgcgcgaat
     1081 tcccaattcg gttcacccgg gaaataattt tgtttaactt taagaaggag atatacatat
     1141 ggcaagaact gcagatatcc atggtaccga gctcggatcc gtcgacaagc ttatcgccaa
     1201 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga
     1261 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     1321 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     1381 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     1441 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     1501 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     1561 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     1621 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     1681 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     1741 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     1801 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
     1861 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     1921 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     1981 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     2041 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     2101 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     2161 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     2221 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     2281 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     2341 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     2401 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     2461 cgctgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     2521 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     2581 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     2641 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     2701 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     2761 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     2821 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     2881 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     2941 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag atctctgttc gggggttcac
     3001 tgacgctgcg tgactacgat ggtcaggttg cggaggctat ggccctggta cgagcgctga
     3061 acaaaatgac gaaagcaggt atgcctgaaa gcgtgcgtat tgcctgaaaa cacaacccgc
     3121 tacgggggag acttacccga aatctgattt attcaacaaa gccactttca gtatggattt
     3181 gggtgttacg ggggggggtt ggtgcttaaa atcgcgctga ggatttctga tggtgttaag
     3241 cgggcggttt tgagatgtaa actcgcccgt ttaacataat ggatcttgcg cgcaccgccc
     3301 gaacaccact cgccacaaaa aaccgccgga acgtccaaaa gtacgggttt tgctgcccgc
     3361 aaacgggctg ttctggtgtt gctagtttgt tatcagaatc gcagatccgg cttcagccgg
     3421 tttgccggct gaaagcgcta tttcttccag aattgccatg attttttccc cacgggaggc
     3481 gtcactggct cccgtgttgt cggcagcttt gattcgataa gcagcatcgc ctgtttcagg
     3541 ctgtctatgt gtgactgttg agctgtaaca agttgtctca ggtgttcaat ttcatgttct
     3601 agttgctttg ttttactggt ttcacctgtt ctattaggtg ttacatgctg ttcatctgtt
     3661 acattgtcga tctgttcatg gtgaacagct ttgaatgcac caaaaactcg taaaagctct
     3721 gatgtatcta tcttttttac accgttttca tctgtgcata tatggacagt tttccctttg
     3781 atatgtaacg gtgaacagtt gttctacttt tgtttgttag tcttgatgct tcactgatag
     3841 atacaagagc cataagaacc tcagatcctt ccgtatttag ccagtatgtt ctctagtgtg
     3901 gttcgttgtt tttgcgtgag ccatgagaac gaaccattga gatcatactt actttgcatg
     3961 tcactcaaaa attttgcctc aaaactggtg agctgaattt ttgcagttaa agcatcgtgt
     4021 agtgtttttc ttagtccgtt atgtaggtag gaatctgatg taatggttgt tggtattttg
     4081 tcaccattca tttttatctg gttgttctca agttcggtta cgagatccat ttgtctatct
     4141 agttcaactt ggaaaatcaa cgtatcagtc gggcggcctc gcttatcaac caccaatttc
     4201 atattgctgt aagtgtttaa atctttactt attggtttca aaacccattg gttaagcctt
     4261 ttaaactcat ggtagttatt ttcaagcatt aacatgaact taaattcatc aaggctaatc
     4321 tctatatttg ccttgtgagt tttcttttgt gttagttctt ttaataacca ctcataaatc
     4381 ctcatagagt atttgttttc aaaagactta acatgttcca gattatattt tatgaatttt
     4441 tttaactgga aaagataagg caatatctct tcactaaaaa ctaattctaa tttttcgctt
     4501 gagaacttgg catagtttgt ccactggaaa atctcaaagc ctttaaccaa aggattcctg
     4561 atttccacag ttctcgtcat cagctctctg gttgctttag ctaatacacc ataagcattt
     4621 tccctactga tgttcatcat ctgagcgtat tggttataag tgaacgatac cgtccgttct
     4681 ttccttgtag ggttttcaat cgtggggttg agtagtgcca cacagcataa aattagcttg
     4741 gtttcatgct ccgttaagtc atagcgacta atcgctagtt catttgcttt gaaaacaact
     4801 aattcagaca tacatctcaa ttggtctagg tgattttaat cactatacca attgagatgg
     4861 gctagtcaat gataattact agtccttttc ctttgagttg tgggtatctg taaattctgc
     4921 tagacctttg ctggaaaact tgtaaattct gctagaccct ctgtaaattc cgctagacct
     4981 ttgtgtgttt tttttgttta tattcaagtg gttataattt atagaataaa gaaagaataa
     5041 aaaaagataa aaagaataga tcccagccct gtgtataact cactacttta gtcagttccg
     5101 cagtattaca aaaggatgtc gcaaacgctg tttgctcctc tacaaaacag accttaaaac
     5161 cctaaaggct taagtagcac cctcgcaagc tcgggcaaat cgctgaatat tccttttgtc
     5221 tccgaccatc aggcacctga gtcgctgtct ttttcgtgac attcagttcg ctgcgctcac
     5281 ggctctggca gtgaatgggg gtaaatggca ctacaggcgc cttttatgga ttcatgcaag
     5341 gaaactaccc ataatacaag aaaagcccgt cacgggcttc tcagggcgtt ttatggcggg
     5401 tctgcggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc
     5461 ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac
     5521 aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag
     5581 cggtccaatg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg
     5641 atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc
     5701 ggaagcgaga agaatcataa tggggaaggc catccagcct cgcg
//
