LOCUS       sequence-132368-         752 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vectors for the salycilate cascade expression system 
            (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm-nasF 
            attenuator-MCSII, M13 replication origin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Medina C, Camacho EM, Flores A, Mesa-Pereira B, Santero E
  TITLE     Improved expression systems for regulated expression in Salmonella 
            infecting eukaryotic cells.
  JOURNAL   PLoS One. 2011;6(8):e23055. doi: 10.1371/journal.pone.0023055. Epub 
            2011 Aug 1.
  PUBMED    21829692
REFERENCE   2  (bases 1 to 752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(8):e23055. doi"; pages: " 
            10.1371/journal.pone.0023055. Epub 2011 Aug 1"
COMMENT     Sequence Label: pMPO 60
FEATURES             Location/Qualifiers
     source          1..752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..24
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5..23
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(525..605)
                     /label=Pm promoter
                     /note="The bacterial Pm promoter is activated by XylS in 
                     the presence of benzoate or m-toluate (Marques et al., 
                     1999)."
ORIGIN
        1 cgcgtaatac gactcactat agggcgaatt ggcgataagc ttgtcgacgg atccgagctc
       61 ggtaccatgg atatctgcag ttcttgccat atgcccgggt gaaccgaatt gggaattcgc
      121 gcaaaaaaaa agcgcccggc ggtgcttacg caccgctgga cgcctttatc cctggacata
      181 cgcgccgcca ttggcgcgct gcccaaaacc ttttattcac tcgaattcag gcattgacga
      241 aggcattcag ctcaccatct ttgttgcgtg tgatgaatat cggctgccgg cccatctgcg
      301 tggtgtaata gtcgttcttc tcgggaatct ggctctcgtg ggcgagataa atccagttgc
      361 cctcaaagat gtgtttcatc tctaaatcga acagccgagg gtcggtgaac atctcgcgct
      421 tgcagcggta gatgccctcg ttctcatctt cttcaacgag actatctata tagtcgagcc
      481 ccaggtgcat tgtcatggtc atgactccat tattattgtt tctgttgcat aaagcctaag
      541 gggtaggcct ttctagagat agccattttt tgcactcctg tatccgcttc ttgcaaggct
      601 ggacttatct ctatcaaatt ccctggcagt ttatggcggg cgtcctgccc gccaccctcc
      661 gggccgttgc ttcgcaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc
      721 gttcaccgac aaacaacaga taaaacgaaa gg
//
