LOCUS       sequence-132370-         705 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vectors for the salycilate cascade expression system 
            (Pnah/NahR-Psal/XylS2).ApR, expression vector with 
            rrnBT1T2-Pm-MCSII, M13 replication origin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Medina C, Camacho EM, Flores A, Mesa-Pereira B, Santero E
  TITLE     Improved expression systems for regulated expression in Salmonella 
            infecting eukaryotic cells.
  JOURNAL   PLoS One. 2011;6(8):e23055. doi: 10.1371/journal.pone.0023055. Epub 
            2011 Aug 1.
  PUBMED    21829692
REFERENCE   2  (bases 1 to 705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(8):e23055. doi"; pages: " 
            10.1371/journal.pone.0023055. Epub 2011 Aug 1"
COMMENT     Sequence Label: pMPO 54
FEATURES             Location/Qualifiers
     source          1..705
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..24
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5..23
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(415..495)
                     /label=Pm promoter
                     /note="The bacterial Pm promoter is activated by XylS in 
                     the presence of benzoate or m-toluate (Marques et al., 
                     1999)."
     terminator      complement(590..676)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cgcgtaatac gactcactat agggcgaatt ggcgataagc ttgtcgacgg atccgagctc
       61 ggtaccatgg atatctgcag ttcttgccat atgcccgggt gaaccgaatt gggaattcag
      121 gcattgacga aggcattcag ctcaccatct ttgttgcgtg tgatgaatat cggctgccgg
      181 cccatctgcg tggtgtaata gtcgttcttc tcgggaatct ggctctcgtg ggcgagataa
      241 atccagttgc cctcaaagat gtgtttcatc tctaaatcga acagccgagg gtcggtgaac
      301 atctcgcgct tgcagcggta gatgccctcg ttctcatctt cttcaacgag actatctata
      361 tagtcgagcc ccaggtgcat tgtcatggtc atgactccat tattattgtt tctgttgcat
      421 aaagcctaag gggtaggcct ttctagagat agccattttt tgcactcctg tatccgcttc
      481 ttgcaaggct ggacttatct ctatcaaatt ccctggcagt ttatggcggg cgtcctgccc
      541 gccaccctcc gggccgttgc ttcgcaacgt tcaaatccgc tcccggcgga tttgtcctac
      601 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag
      661 cctttcgttt tatttgatgc ctggcagttc cctactctcg catgg
//
